利用牙鲆鳃细胞fg和斑马鱼胚胎对氨基甲酸酯类杀虫剂残杀威和西维因的细胞毒性、遗传毒性和致畸性的毒性评估分析-evaluation and analysis of cytotoxicity, genotoxicity and teratogenicity of carbamate insecticides propoxur and carbaryl from paralichthys olivaceus gill cell fg and zebrafish e.docxVIP

Evaluation of Cytotoxicity, Genotoxicity and Teratogenicity of Two Carbamate Insecticides, Propoxur and Carbaryl Using Flounder Gill (FG) Cells and Zebrafish Embryos利用牙鲆鳃细胞 FG 和斑马鱼胚胎对氨基甲酸酯类杀虫剂残杀威 和西维因的细胞毒性、遗传毒性和致畸性的毒性评估研究Date submitted: _______________ Research Guide: ____ (Prof. Zhang Shicui)Approved by (Professors): ____________ ____ ____ ____ ____ ____ ____ 独创声明本人声明所呈交的学位论文是本人在导师指导下进行的研究工作及取得的 研究成果。据我所知，除了文中特别加以标注和致谢的地方外，论文中不包含其 他 人 已 经 发 表 或 撰 写 过 的 研 究 成 果 ， 也 不 包 含 未 获 得（注：如没有其他需要特别声明的，本栏可空）或其他教育机构的学位或证书使 用过的材料。与我一同工作的同志对本研究所做的任何贡献均已在论文中作了明 确的说明并表示谢意。学位论文作者签名：签字日期：年月日---------------------------------------------------------------------学位论文版权使用授权书本学位论文作者完全了解学校有关保留、使用学位论文的规定，有权保留并 向国家有关部门或机构送交论文的复印件和磁盘，允许论文被查阅和借阅。本人 授权学校可以将学位论文的全部或部分内容编入有关数据库进行检索，可以采用 影印、缩印或扫描等复制手段保存、汇编学位论文。（保密的学位论文在解密后 适用本授权书）学位论文作者签名： 导师签字：签字日期： 年 月 日 签字日期： 年 月 日AbstractTwo carbamate insecticides, propoxur and carbaryl, having a wide spectrum of applications, have intensified the risk of exposure to non‐target organisms due to their indiscriminate use. Propoxur (2‐isopropoxyphenyl methylcarbamate) is used throughout the world as insecticides, herbicides, nematocides, acaricides, fungicides, rodenticides, avicides, and bird repellents with their application in a wide variety of habitats including agricultural lands, forests, rangelands, wetlands, residential areas, and commercial sites. Carbaryl (1‐naphthyl‐N‐methylcarbamate), the most frequently used insecticide in the carbamate chemical family, is widely used for the control of a variety of pests on fruits, vegetables, cereals, forage, cotton, forests. lawns, ornamentals and many other crops as well as poultry, livestock and pets. The toxic mode of action of carbamate insecticides for animals is the inhibition of the enzyme acetylcholinesterase (AChE) at synaptic junctions in the nervous system, resulting in the accumulation of acetylcholine in the nerve synapses, causing uncontrolled movement, paralysis, convulsions, tetany, and