利用蛋白质组学技术解析化学杀雄剂sx-analysis of chemical androcide sx by proteomics technology.docxVIP

报道中也提到过化学杀雄剂会使不育系中游离氨基酸的含量下降。通过分析这些差异蛋白，发现还有很多差异蛋白与糖类代谢相关，这表明在化学杀雄剂处理后糖类 代谢的酶会下降表达，这些酶类的下降表达会对能量物质的积累和脂肪酸积累产生 影响，而花粉细胞发育所需要的营养物质与脂肪酸都会受到很大影响，最终可能会 造成甘蓝型油菜育性下降。（3）对差异表达的部分蛋白质进行了表达分析，荧光定量 PCR 分析结果显示 化学杀雄剂处理后，ACTIN12，auxin reponsive-like protein，carboxypeptidase，chalcone synthase 3 protein，Cysteine protease，protein disulfide isomerase precursor-like，protein TAPETUM 1，7 个已经报道的与雄性育性相关蛋白，在花粉发育第 8、9 个两个关键 时期时有 6 个蛋白出现下降表达，这些蛋白的下降表达均会在不同程度上造成甘蓝 型油菜雄性不育的发生。关键词：甘蓝型油菜 雄性不育 化学杀雄剂 蛋白质组学AbstractRapeseed (Brassica napus) is one of the main oilseed crops. The major task for breeders is to increase the seed production at present. Hybrid cultivars have been used to increase the production of rapeseed worldwide successfully. Male sterility of rapeseed induced by chemical hybridization agents (CHA) is one of the main ways to produce hybrid rapeseed. SX-1 as a new-typed CHA had been widely applied to increase hybrid seed production with high efficiency in China. A series of sterile lines that induced bySX-1, such as Y133、Sn338、YD66A had been selected out and new hybrids cultivars whichcontain Qinyou33, Qinyou19, Qinzayou5, Qinrong2 and Qinzayou19 were also cultivated successfully.As far as we known, the proteomic study on male sterile of anthers that induced by CHA in B. napus has not been reported yet.In order to observe the anthers changes after SX-1 treatment in B. napus. Firstly, the development of microspore and tapetum were observed by using transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The results show that, few obvious morphological changes between microspores of control group and SX-1 treatment group in the beginning. All of them seemed to be full and round. However, nearly 100% of treated pollen grains were crimpled and these pollen coats lacked in the late stage of pollen grains in maturation process. Plasmolysis occurred in the crimpy pollen grains. The organelles were not evident and the microspores were almost empty of contents at last. Mea