肝心宁胶囊对肝纤维化HALNPCⅢC-IV影响及机理研究.docVIP

肝心宁胶囊对肝纤维化HALNPCⅢC-IV影响及机理研究 　　摘 要：目的：探讨中药复方肝心宁（GXN）胶囊抗实验性肝纤维化（HF）的疗效及作用机理。方法：以四氯化碳（CCI4）复合因素制作大鼠HF模型，并给予GXN灌胃治疗，同时设秋水仙碱（COL）、大黄虫丸（DHZCP）作为对照。观察经GXN治疗对大鼠肝脏指数、脾脏指数、血清透明质酸酶（HA）、板层素（LN）、IV型胶原（C-IV）、Ⅲ型前胶原（PCⅢ）及病理形态学的影响。结果：本药有一定的回缩肝脾作用；能降低血清HA、LN、C-IV、PCⅢ等HF指标。结论：提示本药有良好的抗HF作用。 　　 　　关键词：肝心宁；肝纤维化；治疗；机理 　　中图分类号：R-33 　　文献标识码：A文章编号： 　　1673-7717（2008）05-1049-03 　　?? 　　Study of Effect and Mechanism of Ganxinning ?Extract on HA LN PCⅢ and C-IV of Hepatic Fibrosis 　　?? 　　GUO Lai?1，WANG Zai?mo?2? 　　（1.Emergency Depcntmcnt，Guanganmen Hospital，China Academy of Chinese Medical Sciences,?Beijing 100053，China；2.Chengdu University of Chinese Medicine，Chengdu 610075，Sichun，China）? 　　Abstract：Objective：To study the effect and mechanism of the Chinese compound prescriptionGanxinning(GXN,a kind of extract of Chinese Traditional herbs, with the functions of resolve blood stasis and nurish Yin,Clear away heat and dampness) extract on experimental hepatic fibrosis. Methods: The models of hepatic fibrosis were produced in Sprague-Dawlay rats by CCl?4 compound factors and were treated with GXN. Colchicin (COL) and Daihuangzhechong pill (DHZCP) were used as control at the same time. The variations of the indexes of hepatis fibrosis including liver index、spleen index、hyaluronic acid (HA)，laminin (LN)，collagen type IV (C-IV) and precollagen typeⅢ (PCⅢ) as well as the variations of the pathomorphology of liver were observed. Results: GXNcould restore the pathological proliferation of livers and spleen of rats to some extent. The serum level of HA，LN，C-IV and PCⅢ were decreased significantly. Conclusion: GXN had obvious inhibitory effect on hepatic fibrosis. 　　 　　Keywords：ganxinnign(GXN) extract；hepatic fibrosis；treatment；mechanism 　　 　　1实验材料? 　　 　　1.1实验动物SD(Sprague-Dawlay)雄性大鼠83只，体重(200±10)g,一级动物，由四川省抗生素研究所提供，检验后备用。? ???　1.2主要试剂液体双试剂（上海科学东菱试剂厂提供）；PCⅢ试剂盒（重庆肿瘤研究所提供）；C-IV试剂盒（北京化学试剂研究所提供）；HA试剂盒（上海海军医学研究所提供）；LN试剂盒（上海海军医学研究所生物技术中心提供）；37％～40％甲醛（重庆东方试剂厂提供，分析纯，批号：950938）；CCI4（郑州化学试剂二厂提供，