微小RNA在直肠癌组织中表达及其临床意义.docVIP

微小RNA在直肠癌组织中表达及其临床意义 　　[摘要] 目的 研究微小RNA（MicroRNA）在直肠癌组织与正常直肠组织中的表达差异。 方法 提取60例直肠癌及癌旁正常组织中总RNA，对标本中MicroRNA表达状况应用实时荧光定量PCR方法进行检测。对其他20例直肠癌组织及其癌旁正常组织应用问题分析（PAM）法进行定性判断。 结果 在直肠癌发生过程中有41种MicroRNA表达量显著下降（＜0.66）。对20例直肠癌组织应用PAM法进行定性判断的结果与病理检查结果基本一致。 结论 MicroRNA与直肠癌联系密切，可以成为直肠癌早期预测和诊断的有效手段。 　　[关键词] 微小RNA；直肠癌症；实时荧光定量PCR 　　[中图分类号] R735.37 [文献标识码] A 　　 [文章编号] 1673-7210（2012）02（b）-0023-03 　　 　　Clinical significance and down-regulative expression of microRNA cluster in human colorectal cancer 　　WU Shangbiao CHEN Rong QUAN Tianyi LI Zhifa 　　Department of Gastrointestinal Surgery, the Third Affiliated Hospital of Guangzhou Medical College, Guangdong Province, Guangzhou 510150, China 　　[Abstract] Objective To investigate the different expression of microRNA in colorectal carcinoma and adjacent ontumorous tissues. Methods Sixty colorectal carcinoma and adjacent non-tumorous tissues were collected respectively and the total RNA was isolated routinely. The microRNA expression was detected by using real-time quantitative polymerase chain reaction (real-time PCR). Problem analysis method was used to quantitatively determine the characteristics (cancer or non-cancer) of the other 20 pair samples. Results There are 47 kinds of microRNAs in cancer were down-regulated (1ess than 0.66). The results of qualitative determination on the 20 samples of cancer tissue/normal tissue were con-sistent with the results of pathological examination. Conclusion Closely linked with the pathogenesis and development of colorectal cancer, MicroRNA may be a promising diagnostic biomarker in diagnosis of colorectal cancer. 　　[Key words] MicroRNA; Colorectal cancer; Real-time PCR 　　 　　MicroRNA（minRNAs）又称微小RNA，是一类不具有蛋白质编码功能的单链RNA，一般长度仅为25个核苷酸左右。近年来研究发现，MicroRNA在生物体中参与许多生理病理过程，并发挥着极其重要的作用。其参与的细胞分裂、分化及凋亡活动中与肿瘤的关系最为密切。 　　直肠癌作为消化道恶性肿瘤极为常见。据统计，近年来我国直肠癌患者明显增多，发病率上升明显，严重威胁人民身心健康。因此，早诊断、早治疗对直肠癌患者的预后改善有着极其重要的意义。 　　以MicroRNA为主要研究方向的癌症靶向治疗及其肿瘤分子标记技术已经成为世界热点课题，具有很广泛的应用前景，但是MicroRNA在直肠癌诱发机制中的作用研究还处于起步阶段[1-2]。随着实