安胃汤对胃溃疡大鼠血清瘦素、tgf-α、no的影响-effects of anwei decoction on serum leptin, tgf - α and no in rats with gastric ulcer.docx

血清中瘦素、TGF-α、NO表达水平明显增强（均P<0.05）；3.在降低溃疡指数、增加再生粘膜厚度、减小粘膜肌层缺损度，提高血清中瘦素、TGF-α、NO表达水平等方面，C组作用均强于D组和E组（溃疡指数、再生粘膜厚度、粘膜肌层缺损度：均P<0.05；瘦素、TGF-α、NO：P<0.05）。结论：1.安胃汤促进了实验性大鼠胃溃疡炎性渗出物、坏死组织的清除，上皮细胞的再生及腺体的重建。2.安胃汤提高胃溃疡大鼠血清中瘦素、TGF-α、NO表达水平，这可能是安胃汤促溃疡愈合的重要机制。3.在促进实验性大鼠胃溃疡的组织修复和提高胃溃疡大鼠血清中瘦素、TGF-α、NO的表达水平方面，安胃汤大剂量组作用明显强于小剂量组，体现出一定的量效关系。4.在促进实验性大鼠胃溃疡的组织修复和提高胃溃疡大鼠血清中瘦素、TGF-α、NO的表达水平方面，安胃汤大剂量组作用明显强于西药奥美拉唑对照组。5.瘦素的表达水平与TGF-α、NO的表达水平呈正相关，瘦素可能通过增加TGF-α和NO促进溃疡愈合。关键词：安胃汤；胃溃疡；LEPTI、TGF-α、NOEffectsofAnweitangonExpressionofSerumLeptin,TGF-α,NOinExperimentalGastricUlcerinRatsABSTRACTObjective:TofurtherexplorethemechanismofAnweitanginimprovinggastriculcerhealingbyinvestigatingtheinfluenceofAnweitangonserumleptin，TGF-α，NOofgastriculcerrats.Methods:50Wistarrats,halfmaleandhalffemale,180±50ginweight,wererandomlyandaveragelydividedintonormalcontrolgroup(groupA),thepathologicalmodelgroup(groupB),Anweitanghighandlowdosagegroup(groupCandgroupDrespectively),Omeprazolegroup(groupE).5teams,eachteamhad10rats.exceptthenormalcontrolgroup,Fortherest40ratsweremademodelsofgastriculcerthroughacidinduction,SalinewasgiventoratsofgroupAandgroupB.Ratsinothergroupweretreatedwithcorrespondingdrugs.After13days,stoppedtreatingandfeedingfor24hours.Adoptedthemethodoffemoralvenousphlebotomize,got4mlblood,putedindrytesttube3000r/min15min,gotsupernatantliquidtoputitin－20refrigeratorforexamination.Adoptedtheradio-immunity,ELISA，NRchemicolorimetrytomakequantitativeanalysisofserumleptin，TGF-α，NOofgastriculcerratsrespectively.Cutcardiaandhelicobacterrespectively,thentookouttherats’gastrictissuessoastocutulcersandadjacent(3mm)tissuesabout1.0×1.2cm,locatedthefociorscarsofulcersandadjacenttissues;fixedthemwith10%formaldehydefor3minutes,calculatedulcerating,thenfixedthemwith10%formaldehydeasusualpracticefor24hours.embeddedparaffinwax,ThepathologyofgastriculcertissueswasobservedwithHEstain.Results:1.ComparedwithgroupA,theexpressionofserumleptin，TGF-α，NOsignificantlyincreasedingroupB,C,DandE(allP<0.05).2.ComparedwithgroupB,ulceratingdec