沉默survivin基因联合低剂量二甲双胍对三阴性乳腺癌细胞生长抑制和凋亡诱导影响-effect of silent survivin gene combined with low dose metformin on growth inhibition and apoptosis induction of triple negative breast cancer cells.docxVIP

PAGE 5 PAGE 5 combined RNAi-mediated survivin gene silencing with low dosese of metformin for further experiments and clinical practice. Methods: ① according to the cDNA sequence of the survivin gene ,design and synthesis the shRNA which specified silencing the survivin gene ,and construct the recombinant plasmid .It will be identified by restriction enzyme digestion and sequencing .Then, the recombinant plasmids were extracted and transfected into the MDA-MB-231 cells. G418 screening to obtain the stable transfected cells, MTT detection the growth inhibition of the stable transferred cells