番茄slasa1基因的克隆及功能研究-cloning and functional study of tomato slasa 1 gene.docxVIP

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番茄slasa1基因的克隆及功能研究-cloning and functional study of tomato slasa 1 gene.docx

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番茄slasa1基因的克隆及功能研究-cloning and functional study of tomato slasa 1 gene

ABSTRACTAuxins play a pivotal role in various aspects of plant growth and developmental processes such as cell division, extension, and differentiation, apical dominance, tropism movement, embryonic development, laterals formation, and vascular differentiation, via regulating the expression of early auxin responsive genes.Tomato (Solanum lycopersicum) is a ideal model plant for fruit development research, which have many specific characteristics such as: small genome size, Gene homozygote from self-pollination, short growth cycle, easy plant in green house and existing genetic transformation system. Therefore, in order to research the function of Auxin in plant and fruit development, we utilize tomato as raw material.ASA1 gene expresses a rate limit enzyme element (Anthranilate synthase component I), which catalyze the reaction of Anthranilate (auxin precursor) synthesis. Meanwhile, as already confirmed in Arabidopsis, ASA1 gene is the interaction node ofAuxin and Ethylene. It play an important role in plant，via regulating the Auxinsynthesis.Aiming at reveal the role of SlASA1 by auxin signaling regulation in plant, the study utilizes tomato as material clone and identified the two genes, individually. Then, by expression pattern analysis, plant hormonal stimulation, and promoter-GUS analysis, the study further understand the expression specific of the two genes. Meanwhile, the study constructs the gene overexpression vector and suppress vector of SlASA1. By transforming these vector into tomato, the gene overexpression and suppress lines was obtained, and then was used to observe the phenotype that was caused by gene expression diversity. Finally, we have some results as follow:1）Based on the AtASA1 gene sequence (NM_001203302.1), an AtASA1-like gene(SGN-U573188) was isolated from SGN () and designated as SlASA1. According to the sequence of SlASA1, the length is 1929 bp contain a 1347 bp open read frame (ORF) that encodes a polypeptide of 448 amino acids