缺血性中风病中医证候规律及血瘀证蛋白质组学分析-tcm syndrome regularity and proteomic analysis of blood stasis syndrome of ischemic stroke.docxVIP

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缺血性中风病中医证候规律及血瘀证蛋白质组学分析-tcm syndrome regularity and proteomic analysis of blood stasis syndrome of ischemic stroke.docx

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缺血性中风病中医证候规律及血瘀证蛋白质组学分析-tcm syndrome regularity and proteomic analysis of blood stasis syndrome of ischemic stroke

AbstractObjective:Toinvestigateischemicstrokepatternregularityandthecorrelationsbetweenbloodstasissyndromeandproteomics.Method:ThediagnosisofcerebralinfarctionanditshypotypeisaccordingtotheDiagnosingCriteriaofcerebralVascularDiseasepublishedbyChinaNeurologyAssociationin1995.Allthediagnosisofthepatientsareconfirmedbyimaging.Thesubjectsagedinbetween40-85years,onsetedtimewithin3days.TheTCMsyndromediagnosisisbasedontheTCMSyndromeDiagnosticCriteria.Wegatherthegeneralstateofhealth,incidence,pastmedicalhistory,laboratoryexamination,thequantizationscoreoftheTCMsyndrome,takethebloodsamples,usingthetwo-dimensionalelectrophoresisandmassspectrometrytoseparateandidentifyitsproteins.Results:Thereare13cases(61.90%)establisheddiagnosisofbloodstasissyndromeinmalepatients;1case(11.11%)establisheddiagnosisofbloodstasissyndromeinfemalepatients,P=0.011,theincidencebetweenmaleandfemalepatientshaveasignificantdifference,showingthebloodstasisincidenceinmalepatientswashigherthanfemalepatients.Hotsyndrome(with85.17%incidencerateinmalepatients,with55.56%incidencerateinfemalepatients)andhyperactivitysyndrome(57.14%incidenceofmalepatients,22.22%incidenceoffemalepatients)Pvalueofthetwosyndromeswasslightlyhigherthan0.05,thestudyalsoconsideredthattheincidenceratehavingclinicaldifferences;Thereare14cases(100%)establisheddiagnosisofphlegmdiagnosisinpatientsdown60years;8cases(50%)establisheddiagnosisofphlegmdiagnosisinpatientsover60years,P=0.002;11(78.57%)casesestablisheddiagnosisofbloodstasisinpatients≤60years,3cases(18.75%)establisheddiagnosisofbloodstasisinpatientsover60years,P=0.001;10cases(71.43%)establisheddiagnosisofhyperactivitysyndromeinpatientsdown60years,4patients(25%)establisheddiagnosisofhyperactivitysyndromeinpatientsover60years,P=0.011;theincidencehaveasignificantdifference,showingthephlegm,bloodstasisandhyperactivitysyndromeincidenceinpatientsdown60yearswashigherthanpatientsover60years;Hotsyndrome(with92.86%incidencerateinpatientsdown60years,with62.5%incidencerateinpatientsover60years