shkbp1等几种基因工程小鼠纯合品系建立-establishment of homozygous strains of sh kbp 1 and other genetically engineered mice.docx
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shkbp1等几种基因工程小鼠纯合品系建立-establishment of homozygous strains of sh kbp 1 and other genetically engineered mice
toinflammation,cancerandtheroleofvasculardiseasebycrossingtoApoEKO,Rip1-Tag2,ApcMin,TRAMPandMMTV-PvMTmiceandotherdiseasedmicemodel.MethodBreedinggeneticengineeringmiceGeneticallyengineeredmicewerehousedinSPF-classenvironment,accordingtotheneedforbreeding,allocatingmicetimelyafterlactation,tomakepreparationforthescreeningofhomozygousmice.TheestablishmentofstablegeneticallyengineeredmiceandscreeningofhomozygousAppliedclassicMendelianlawsingeneticstobreedthetransgenicmice,andcombinedwithPCRtodetectthetargetgenes.Shkbp1geneontumorgrowthMakingShkbp1homozygousknockoutmiceastheexperimentalgroup,wild-type129miceasthecontrolgrouptoestablishsubcutaneousB16melanomatumormodelMeasuringthetumorvolume,tumorweight,tofurtherinvestigatetheeffectofShkbp1knockoutmiceontumorgrowthandmetastasis.Results129generationmicecrosswithF2positivemicetodetectthegenotypesoftheiroffspring.whenonemaleandthreefemaleShkbp1micehavebeenselected,theoffspringgeneidentificationresultswas100%;whentwomaleandonefemaleSGTamicehavebeenselected,theoffspringgeneidentificationresultswas100%;whenonemaleandonefemalepVA-hCD2-Nefinmicehavebeenselected,theoffspringgeneidentificationresultwas100%.Rip-hSlit2mousefilterisstillinprogress.Shkbp1insubcutaneoustumorandmetastatictumormicemodel(theresultsareinstatistics)ConclusionShkbp1homozygousknockoutmiceweresuccessfullyconstructed,anditisverifiedtheeffectofShkbp1geneintumorgrowthandpreliminarymetastasis.SGTahomozygousknockoutmiceweresuccessfullyconstructed.GenerationF2pVA-hCD2-NefandgenerationF1Rip-hSlit2TGmicewereconstructed.Keywords:geneticallyengineeredmice;homozygous;Shkbp1;SGTa;nef;Rip-hSlit2;广东药学院学位论文原创性声明本人郑重声明:本人所呈交的学位论文,系我个人在导师的指导下进行研究工作所取得的成果。除文中已特别加以标注和致谢的地方外,不包含其它个人或机构已经发表或撰写过的研究成果。对本研究做出贡献的其它个人和集体,均已在文中明确说明和致谢。本人充分意识到本声明的法律结果完全由本人承担。学位论文作者签名:____日期:年月日学位论文使用授权的声明本人完全了解广东药学院有关保留和使用学位论文的规定,学校有权保留和向有关部门或机构送交本论文的复印件和电子版,允许论文被查阅和借阅。学校可以将本学位论文的全部或部分内容编入有关数据库,可以采用影印、缩印或其它复印手段保存和汇编本学位论文。保密论文在解密后适用本声明。论文作者签名:_____论文导师签名:_____日期:年月日第一部分Shkbp1等几种基因工程小鼠纯合品系的建立引言人类疾病的动物模型(an
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