gfp基因标记的杧果胶孢炭疽菌的特性分析-analysis of characteristics of pectin colletotrichum gloeosporioides labeled with gfp gene.docx
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gfp基因标记的杧果胶孢炭疽菌的特性分析-analysis of characteristics of pectin colletotrichum gloeosporioides labeled with gfp gene
2.1 病样症状与分离株的菌落形态..................................................................................162.2 分离株的分生孢子及附着胞......................................................................................162.2.1 孢子的形成及其大小.......................................................................................162.2.2 孢子萌发及附着胞的形成...............................................................................172.3 分离株的分子鉴定......................................................................................................172.3.1 PCR 鉴定分离株182.4.2 rDNA-ITS 区序列比对182.4 分离株的致病性..........................................................................................................193 小结与讨论.............................................................................................................................20 第三章杧果炭疽病菌遗传转化体系的构建.................................................................................21 1.材料与方法 .............................................................................................................................211.1 实验材料.......................................................................................................................211.2 方法..............................................................................................................................221.2.1 原生质体制备....................................................................................................221.2.2 质粒的提取........................................................................................................231.2.3 质粒验证...........................................................................................................24DNA 的转化24MCGW 与 MCGT 的抗性鉴定24MCGT 的分子鉴定25MCGT 的荧光检测25MCGT 的荧光检测25MCGT 的生长稳定性分析252 结果与分析.............................................................................................................................252.1 原生质体的收集....................................................................................................
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