正交设计法优选银杏叶黄酮类化合物提取工艺研究.docVIP

正交设计法优选银杏叶黄酮类化合物提取工艺研究 　　[摘要] 目的:采用正交设计法对银杏叶黄酮类化合物提取工艺参数进行优化。方法:通过以分光光度法测定黄酮含量为指标,以固液比、提取温度、浸取剂pH值为考察因素,采用正交试验优选最佳提取工艺。结果:最佳提取工艺为固液比1∶15、提取温度70℃、浸取剂pH值为8。结论:该工艺稳定、合理、可行,适用于工业化生产。 　　[关键词] 正交设计;银杏叶;黄酮类化合物;提取工艺 　　[中图分类号] R284.2[文献标识码]C[文章编号]1674-4721(2010)06(a)-134-02 　　 　　Optimization of the extration of technology of the flavonoids from leaves of Ginkgo biloba by orthogonal design 　　CHEN Dengfeng1, LI Liehui2, LUO Maoyu3 　　(1. The Peoples Hospital of Nanping, Fujian Province, Nanping 353000, China; 2. Shouning County Hospital, Fujian Province, Shouning 355500, China;3. The First Hospital in Subsidiary Longyan of Medical University in Fujian, Longyan 364000, China) 　　[Abstract] Objective: To optimize the parameters of the flavonoids from leaves of Ginkgo biloba by orthogonal design. Methods: The extraction technology was optimized by orthogonal experiment with the content of flavonoids which was determined by spectrophotometry, and it was indexes while with ratio of solid to liquid, leaching temperature and pH. Results: The optimal extraction technology was as follow: the ratio of solid to liquid was 1∶15, leaching temperature was 70℃ and pH was 8. Conclusion: This extraction technology is stable, reasonable and feasible, and it is applicable for experimental studies. 　　[Key words] Orthogonal design; Leaves of Ginkgo biloba; Flavonoids; Technology on extract 　　 　　银杏叶为银杏科植物银杏的干燥叶,其主要化学成分为黄酮类和萜内酯类化合物。银杏黄酮类化合物可以扩张血管,增加冠状动脉及脑血管流量,降低血黏度,改善微循环,是治疗心脑血管疾病的有效药物。萜内酯是血小板活化因子(PAF)受体特异性拮抗剂[1]。银杏叶中黄酮类化合物的提取率直接决定银杏叶的药用价值,因而银杏叶的提取工艺已成为国内外的研究热点[2-6]。本文以银杏总黄酮的提取率为指标对银杏叶提取物的提取和精制工艺进行研究,根据实际生产条件,采用70%乙醇为提取剂,对影响浸取的主要因素进行了研究,旨在提高银杏总黄酮的提取???及产品的质量。 　　1材料与方法 　　1.1 仪器与试剂 　　紫外分光光度计:UV-2540(岛津)紫外分光光度计;芦丁(化学对照品)批号:080-9303,中国药品生物制品检定所提供;乙醇、硝酸铝、氢氧化钠、亚硝酸钠等试剂均为AR级。 　　1.2 实验方法 　　1.2.1 银杏总黄酮含量测定参照文献[7],采用分光光度法。 　　1.2.2 对照品溶液的制备精密称取经105℃干燥至恒重的芦丁对照品10.5 mg,置50 ml量瓶中,加70%乙醇适量,置水浴箱上微热使其溶解,放置冷却,加70%乙醇定容至50 ml,摇匀,即得(每毫升中含无水芦丁0.21 mg)。 　　1.2.3 空白对照溶液的制备取20 ml量瓶,加5%亚硝酸钠溶液1 ml,混匀,放置6 min,加1