正常和羟多巴胺毁损大鼠脚桥核神经元对胆碱能M受体刺激的反应的论文.docVIP

正常和6羟多巴?胺毁损大鼠脚桥核?神经元对胆碱能M?受体刺激的反应的?论文 正常和6?羟多巴胺毁损大鼠?脚桥核神经元对胆?碱能M受体刺激的?反应的论文 ?【摘要】 ? 目的 在体水?平上观察选择性胆?碱能m受体激动剂?氧化震颤素(ox?om)对正常和?6羟基多巴胺(?6ohda)毁?损大鼠脚桥核(p?pn)神经元自发?电活动的影响。方?法 采用玻璃微电?极细胞外记录和p?pn内注射oxo?m，观察ppn?神经元电活动的变?化。结果 与正常?组大鼠相比，6?ohda毁损组大?鼠ppn神经元的?平均放电频率增高?(p 0.01)?，神经元活动趋于?不规则(p 0.?001);ppn?内注射oxom?对正常组和6o?hda毁损组大鼠?的ppn神经元放?电频率均可产生升?高、降低和无变化?三种改变。然而，?ppn内注射ox?om对正常组大?鼠ppn神经元的?平均放电频率无明?显影响，但oxo?m使6ohd?a毁损组大鼠pp?n神经元的平均放?电频率显著降低(?p 0.001)?。结论 胆碱能m?受体激动剂抑制损?毁组大鼠ppn神?经元的活动，其作?用机制是复杂的，?涉及到ppn内突?触前和突触后胆碱?能受体。 ? 【?关键词】 ? ? 脚桥核;m型胆?碱能受体;氧化震?颤素;帕金森病;?电生理学 ab?stract: ?objectiv?e to exp?lore the? effects? of oxot?remorine? m (oxo?m), musc?arinic a?cetylcho?line rec?eptor ag?onist, o?n sponta?neous fi?ring rat?e of neu?rons of ?peduncul?opontine? nucleus? (ppn) i?n normal? and 6h?ydroxydo?pamine (?6ohda)?lesioned? rats.me?thods ch?ange in ?the firi?ng activ?ity of p?pn neuro?ns was o?bserved ?by extra?cellular? recordi?ng in vi?vo and l?ocal inj?ection o?f oxom ?in the p?pn.resul?ts the r?esults s?howed th?at the m?ean firi?ng rate ?of ppn n?eurons i?n the le?sioned r?ats was ?signific?antly in?creased ?pared to? that in? normal ?rats (p ?0.01), a?nd that ?the firi?ng patte?rn of th?e neuron?s change?d toward? a more ?irregula?r firing? after t?he lesio?n (p 0.0?01). int?rappn i?njection? of oxo?m induce?d increa?se, decr?ease or ?no chang?e in the? firing ?rate of ?ppn neur?ons in b?oth the ?normal a?nd 6ohd?alesion?ed rats.? however?, the me?an firin?g rate o?f ppn ne?urons in? normal ?rats rem?ained un?altered ?by the s?timulati?on of m ?receptor?s, but t?he mean ?firing r?ate of p?pn neuro?ns in th?e lesion?ed rats ?decrease?d signif?icantly ?by intra?ppn inj?ection o?f oxom ?(p 0.001?). concl?usion m ?receptor? agonist? inhibit?s the ac?tivity o?f the pp?n neuron?s in les?ioned ra?ts; the ?action m?echanism? of the ?agonist ?is plica?ted, inv?olving n?ot only ?postsyna?ptic but? also pr?esynapti?c m rece?ptors lo?cated in? differ