加味四逆散对抑郁大鼠大脑海马5-ht2ar及其mrna表达的干预-intervention of jiawei sini powder on 5 - ht2ar and its mrna expression in hippocampus of depressed rats.docxVIP

The intervention of Jiaweisinisan on 5-HT2AR and mRNA of hippocampus of brain in rats on depressionZhang Youwei (Clinical Basis of Traditional Chinese Medicine)Xie ZhongliAbstractObjective: Based on the etiology and pathogenesis to depression, combined with the early clinical observation and animal studies.in treatise on febrile disease, based Sinisan to plus by Treatise on Febrile Diseases of zhongjing.It was effected the cats depression behavior and the hippocampus 5-HT2AR and 5-HT2ARmRNA expression to FenYang and long-term inverse scattered unforeseeable moderate intensity stress , compared with fluoxetine, To investigated intervention neurological basis on Jiaweisinisan to FenYang and long-term inverse scattered unforeseeable moderate intensity stress.To provided theoretical basis and experimental basis on Jiaweisinisan.Methods：The rats were divided into 6 group in randomly, model group, low dosegroup of Jiawei Sini Decoction, the middle dose group of Jiawei Sini Decoction, flushing dose group of Jiaweisini Decoction, fluoxetine group, each Section 12. In addition to the control group, each group received a different stress in 42 days, including cold water swimming, heat stress, to prohibit water stimuli etc. Using Open-field method rat to behavior test,Using test for sugar consumption, To observed sugar consumption. It were used in traditional Chinese medicine Jiawei Sini Decoction or fluoxetine intervention to the four groups after 21 days.the rats were sacrificed at 42 days. to obtain the brain. It were used in Immunohistochemistry and situ hybridization method to expression of rat hippocampus 5-HT2AR and 5-HT2ARmRNA.Results：The model replicated in 21 days, the score of model rats is lower inOpen-Field,and reducing sugar consumption, It is decreases on the overall activity, and listlessness, fur dirt. With the control group, the score of rats is decreased on vertical,horizontal points and sugar water consumption,with a statistical significance. After treat