sp1 klf4和p21在卵巢浆液性腺癌中的表达及意义的 研究-expression and significance of sp1 kl f4 and p21 in ovarian serous adenocarcinoma.docxVIP

目录中文摘要·············································································1英文摘要·············································································6英文缩写·············································································14研究论文SP1、KLF4和P21在卵巢浆液性腺癌中的表达及意义的研究前言·············································································15材料与方法····································································16结果·············································································20附图·············································································25附表·············································································35讨论·············································································38结论·············································································45参考文献·······································································46综述SP1的研究现状····························································51致谢···················································································60个人简历·············································································61SP1、KLF4和P21在卵巢浆液性腺癌中的表达及意义的研究摘要背景：卵巢恶性肿瘤病死率高、预后差，严重威胁了女性的生命和健康。其中上皮性恶性肿瘤占85%~90%，卵巢浆液性癌是卵巢上皮性恶性肿瘤中最常见的一种，其预后与临床期别、病理类型、细胞分化程度、术后残存癌灶的大小及肿瘤细胞对化疗的敏感性有关。卵巢上皮性癌的发病学说——二元论，已被广泛接受及认可，根据卵巢癌二元论学说，将浆液性卵巢癌分为I型和II型。I型卵巢癌分化程度高，恶性程度低，主要是来自卵巢交界性肿瘤和子宫内膜异位，镜下常见良性-交界性-癌的过渡，主要包括：低级别浆液性癌、低级别子宫内膜癌、透明细胞癌及黏液性癌等。II型卵巢癌，分化程度低，恶性程度高，多来源于输卵管上皮，易于早期转移，主要包括：高级别浆液性癌、低分化子宫内膜样癌、未分化癌及癌肉瘤等。因为卵巢癌的早期诊断对其预后很关键，所以目前找到特异的诊断指标或有效的治疗方案是治疗卵巢癌的首要任务。转录因子是一种具有特殊结构的蛋白质分子，能够行使调控基因表达的功能。SPl/Krüppel样转录因子(Spl-likeandKrüppel-liketranscriptionfactors,SP1/KLFs)参与了细胞的增殖与分化。SP1是第一个被克隆的Sp/KLF家族中的转录因子，在哺乳动物细胞中广泛表达，目前关于SP1的研究已经有很多，SP1已作为促癌蛋白被大家所熟知，但其在肿瘤发生、发展过程中的作用还未完全清楚，它在浆液性卵巢癌中的表达情况还有待进一步研究。Krüppex样锌指转录因子(Krüppel-liketranscriptionfactors,KLFs)是SPl/Kruppel样转录因子家族中的一个亚家族，KLFs对不同基因启动子的GT盒具有不同的亲和力，因此KLFs因子在不同组织中既可作为转录激活因子，也可作为转录抑制子。KLFs在细胞增殖、分化、调亡中起重要作用。KLF4在人类组织中广泛表达，其与SP1在锌指的C-端具有