运动区久留针对局灶性脑缺血大鼠血管生成素-1表达影响.docVIP

运动区久留针对局灶性脑缺血大鼠血管生成素-1表达影响 　　 摘 要：目的：观察运动区久留针对局灶性脑缺血模型大鼠脑组织中血管生成素-1(Ang-1)表达的影响。方法：随机选取66只Wistar雄性大鼠采用改良线栓法建立局灶性脑缺血大鼠模型，43只大鼠复制成功模型后随机分为假手术组(14只)、模型组（14只）、久留针组（14只）、短留针组（15只），并设假手术组(14只)进行对照研究，采用改良线栓法建立局灶性脑缺血大鼠模型，选取大鼠运动区进行针刺留针治疗，久留针组留针10h，短留针组留针30min，每天1次，治疗2周，模型组及假手术组不作针刺治疗。对各组大鼠的神经功能进行评分，并用免疫组化法检测血管生成素(Ang-1)的表达。结果：与模型组比较，久留针组的Zealonga评分及平衡木行走试验评分减少(P 　　 关键词：头皮针；运动区；Ang-1；局灶性脑缺血；Wistar大鼠 　　中图分类号：R285.5 文献标识码：A 文章编号：1673-7717(2012)01-0079-03 　　Effect of Long Time Retention of Needle on Angiogenin-1 Expression of Motor-area in Brain Tissue of Rat with Focal Cerebral Ischemia 　　BAO Ye?hua, ZENG You?hua,WANG Yan?wu, WANG Chong?min, CHU Jia?mei 　　(Hangzhou Hospital of TCM, Hangzhou 310007, Zhejiang, China) 　　Abstract:Objective: To observe the effect of long time return needle on motor area on angiogenin-1 in the brain tissue of the rat after focal cerebral ischemia reperfusion. Methods: 66 Male Wistar rats were used to prepare the focal cerebral ischemia reperfusion model by filment occlusion. After the model had been made, 43 rats were randomly divided into four groups: sham operation group,Model group (n=14), Long time return needle group(n=14), Short time return needle group(n=15). Sham Operation Group (n=14) was arranged at the same time. A Wistar rat model of focal cerebral ischemia reperfusion was made by filament occlusion. Motor area was selected as the treatment point. Long-return Needle group was returned the needle for 10 hours and shortShort-return needle group for 30 minute per day for 2 weeks. No special treatment was used on the rats of the other groups. The neurological scores were observed before and after treatment. Angiopoietin- 1 was detected with by immunohistochemical method. Result: After 2 weeks, the neurological scores of long-return needle group were higher compared with model group(P 　　假手术组予颈前正中线切开，并分离右侧颈总动脉、颈内动脉、颈外动脉，缝合，单笼饲养，自由饮水，常规喂养。 　　1.4 治疗方法 久留针组：参照中国针灸学会实验针灸委员会实验动物穴位图谱[6]，取大鼠头穴“运动区”作为刺激部位。选用江苏省吴江市佳辰针灸器械有限公司生产的直径为