荧光定量PCR技术的原理及其应用PPT.ppt

DNA Concentration Measurement The Rotor-Gene is fully equipped to do DNA concentration measurement using fluorescent dyes Using a standard run protocol and integrated analysis software, the concentration of unknown samples is determined from a standard curve. A DNA standard curve with replicates is shown. Curve interpolated using a spline curve fit (Rotor-Gene analysis software). Data was obtained using reagents in the Quant-iT? PicoGreen? dsDNA Kit (Invitrogen Corp., Carlsbad CA). Standard Rotor-Gene concentration analysis run protocol was used. 10 μL PicoGreen? (diluted1/200 in 1? TE buffe