酶标仪快速测定抗菌物质抑菌活性方法地建立.pdfVIP

Advances in Microbiology 微生物前沿, 2014, 3, 29-35 Published Online June 2014 in Hans. /journal/amb /10.12677/amb.2014.32004 Establishment of Rapid Determining Method for Antibacterial Activity by Microplate Reader 1 2 2 2* Zixiong Zhou , Qinhua Huang , Shuang Zhu , Lin Zhou 1 School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 2 Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou * Email: zzxyaoji@126.com, bio_zhoulin@163.com rd th th Received: Apr. 23 , 2014; revised: May 28 , 2014; accepted: Jun. 4 , 2014 Copyright © 2014 by authors and Hans Publishers Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). /licenses/by/4.0/ Abstract To investigate the main factors influencing the antimicrobial activity by microplate reader method for high throughput screen antimicrobial substances, the inhibitory rate of staphylococcus aureus was determinated against four typical antibiotics (kanamycin hydrochloride, terramycin, ampicillin, vancomycin) under different culture conditions. The result showed that the concentration of the drug, culture conditions and culture time will affect the inhibitory rate of Staphylococcus aureus. With bacterial concentrations of 5 × 105 cfu∙mL−1, static culturing, incubation time of 6 h, the inhi- bitory rate of kanamycin hydrochloride, terramycin, ampicillin, vancomycin was 57.4%, 52.8%, 57.4%, 52.8% respectively with the concentration of