最新基因组编辑技术CRISPRCAS_system课件.pptxVIP

Liang Jiwang2015.02.02Fighting invasion. When viruses (green) attack bacteria, the bacteria respond with DNA-targeting defenses that biologists have learned to exploit for genetic engineering.Lactic acidLactoseInvasion. Streptococcus thermophilus (嗜热链球菌) such as this one may acquire key defensive sequence from infectious bacteriophage, attached at top. 发现Clustered Regularly Interspaced Short Palindromic Repeats免疫过程PAM: proto-spacer adjacent motifs(保守的间隔相邻基序)crRNA: CRISPR RNAtracrRNA: trans-activating CRISPR RNA人工改造PAM(一般为NGG)工作原理HDR: Homology-direct repair 同源直接修复NHEJ : Non-homologous end joini