分子生物学第一章--绪论PPT.ppt

分子生物学与基因操作技术;生物学：研究生命现象、生命本质、生命活动及其规律的科学。;细胞生物学：从细胞水平 理解生命活动 遗传学：从遗传角度理解生命活动 生物化学：从化学组成角度来理解生物大分子和生物代谢。 普通生物学（动物植物） 微生物学：不同生物类型的特点 分子生物学：从分子水平理解生命活动;基因工程的发展;　　通过研究“基因敲除”的耗子将帮助研究人类的癌症、糖尿病和高血压等慢性疾病与遗传的关系。 ;教材: 朱玉贤等，现代分子生物学 ，第三版 References: Sambrook J, et al. Molecular cloning-A Laboratory manual. 3rd ed. 2001, Cold Spring Harbor Laboratory Press Technology Press ;Gene Engineering;General Syllabus;Chapter 4 Enzymology of Gene Manipulation（2 hours） Chapter 5 PCR techniques（2 hours） 1????? Principle of PCR techniques 2????? RT-PCR 3????? NEST-PCR 4?????Application of PCR techniques in evolution research or related, such as RFLP,RAPD,SSAR,SNP etc. Chapter 6 Molecular Hybridization (4 hours) 1????? Southern blot 2????? Northern blot 3????? Fluorescence in situ hybridization 4??????Yeast two-hybrid;Chapter 7 Vector Construction and Gene Transformation (4 hours) 1????? Construction of co-expression vector 2????? Vector construction of fusion Protein 3????? Gene transformation and screening 4?????? Safety assessment of gene transformation Chapter 8 Protein Expression (4 hours) 1????? Procaryotic expression system 2????? Eucaryotic expression system 3??????SDSD-electropheris 4????? Antibody preparation 5????? Western blot ;Chapter 9 Introduction of Bioinformatics (2 hours) 1????? Principle and prevalent softwares 2??????Available database on website Chapter 10 Strategy of Gene Cloning (4 hours) 1????? Map-based cloning 2??????Transposon tagging cloning 3????? Gene cloning based on known protein 4 Cloning of Homologous Genes ;Chapter 11 Function Identification of Novel Genes (3 hours) Chapter 12 Application of Gene Engineering (4 hours) Practical application of gene engineering in chemical technology, pharmacy, agriculture, energy resource, environmental protection and materials ;Chapter 13 Guide to Scientific Literature (4 hours) Selected from distinguished periodicals such as Nature, Science, Cell, Pnas, Jacs, Agrew, Plos Biology, etc. ;生命科学主要网址;生命科学主要刊物;