马铃薯茎段愈伤组织诱导及再毕业论文.docVIP

学士学位毕业（论文） 学生姓名： 指导教师： 所在学院： 专 业：20094011302 中国·大庆 201 年 5 2,4-D。选择苗龄合适的马铃薯无菌试管苗，切成0.5厘米长，不带腋芽茎段，接种于含不同浓度细胞分裂素6-BA和生长素2,4-D的愈伤组织诱导培养基，进行诱导培养。愈伤组织每2周继代1次。选择生长良好的愈伤组织，接种苗在含有不同浓度6-BA，NAA和GA的培养基中进行茎段愈伤组织分化培养。结果表明：克新13号的最佳愈伤组织诱导培养基为MS+2mg/L 6-BA +2 mg/L 2,4-D；早大白的最佳愈伤组织诱导培养基为MS+1 mg/L 6-BA+2mg/L 2,4-D。在上述最佳诱导培养基中培养，俩种愈伤组织诱导率分别为62.22%和60.37%，在上述最佳分化培养基中培养，所得的分化率分别为53.81%和57.42%，早大白的分化率高于克新13号的分化率。 Potato Stem Section Callus Induction and Regeneration Student :Guo Yongfeng Supervising: Jiang lili Abstract: Potato stem section callus induction and regeneration of this test in 2012 in agronomy courtyard potato laboratory. To g new 13, the early two potato stem section as materials, all kinds of callus induction and regeneration. Test using reagents: MS medium as the basic medium, the movement of the adjustable plant hormones have 6 - BA, 2, 4-d. Select age of seedling and d potato sterile tube seedlings, cut into 0.5 cm long, dont take lateral bud stem section, vaccination to contain different concentration cytokinins 6 - BA and auxin 2, 4-d of callus induction medium, induction training. Callus every 2 weeks successive transfer 1 time. Select growth good callus, vaccination to contain different degrees of cytokinins 6 - BA and NAA and callus induction of GA3 in medium culture. The results showed that: g new number 13 optimum callus induction medium for MS + 1 mg/L 6 - BA + 2 mg/L 2, 4-d; The early for MS + 2 mg/L 6 - BA + 2 mg/L 2, 4-d. With the above optimum induction medium culture, all kinds of callus induction rate were 62.22% and 60.37% respectively g new, with the optimum induction medium culture, all kinds of differentiation rate were53.81% and 57.42% respectively. Key words: Potato; Callus induction; regeneration 目 录 摘要 I Abstract II 前 言 IV 1 材料与方法 1 1.1 材料 1 1.2 试验设计 1 1.2.1 基础苗培养 1 1.2.2 培养基配制 1 1.2.2.1 MS培养基 1 1.2.2.2 茎段愈伤组织诱导培养基 1 1.2.2.3 茎段愈伤组织分化培养基 2 1.2.3茎段愈伤组织诱导 2 1.2.4 愈伤组织分化 2 2 结果与分析 3 2.1 不同激素配比对马铃薯愈伤组织诱导率的影响 3 2.2 不同激素配