脑损伤大鼠胫骨骨痂中降钙素基因相关肽的改变_论文.docxVIP

脑损伤大鼠胫骨骨痂中降钙素基因相关肽的改变 【摘要】 目的 研究脑损伤后大鼠胫骨骨痂中降钙素基因相关肽(CGRP)的变化及骨痂量的改变。方法 将100只雄性Wistar大鼠随机分为两组，脑损伤加右胫骨骨折组和单纯右胫骨骨折组各50只。术后3、7、14、21、28 d分批处死，检查血清碱性磷酸酶，摄右胫骨X线片测量骨痂面积，骨痂行苏木精2伊红(HE)染色和降钙素基因相关肽免疫组织化学染色。结果 脑损伤加骨折组较单纯骨折组术后3、7 d，碱性磷酸酶均显著升高(P＜)；脑损伤加骨折组较单纯骨折组术后14、21 d，骨痂X线面积大；脑损伤组早期形成大量纤维骨痂和软骨骨痂，骨痂中降钙素基因相关肽免疫阳性神经纤维多，明显增厚的骨膜内层骨祖细胞、幼稚的软骨细胞胞浆内降钙素基因相关肽强阳性表达；骨折愈合快，降钙素基因相关肽表达明显增强。结论 脑损伤后骨痂中降钙素基因相关肽有显著改变，并引起骨痂量和质的改变，推测降钙素基因相关肽参与调节骨折愈合过程。 【关键词】 脑损伤；胫骨骨折；降钙素基因相关肽；大鼠 The Change of Calcitonin Generelated Peptide in Tibial Fracture Callus After Bran Injury in Rats Abstract：Objective To study the relationship between the changes of calcitonin generelated peptide in tibial fracture callus and yield of callus after brain injury in One hundred male Wistar rats were randomly divided into brain injury plus tibial fracture group (n＝50) and tibial fracture group (n＝50).The animals were sacrificed in batches 3，7，14，21 and 28 days after concentration of ALP in serum of rats were ray plane films of the fractured tibial were taken to measure area of were subjected to HE staining and CGRP immunohistochemical Concentration of ALP in serum of brain injury group were significantly higher than those in tibial fracture group at 3 d and 7 d (P＜).The area of callus in brain injury group was larger than in tibial fracture group at 14 d and 21 were a quantity of fibrous callus and cartilaginous callus formation and more CGRP immunoreactive nerve fibers in callus at early stage after brain immunoreactivity of CGRP was found in osteofenitor cells，chondroblasts，which proliferated in thickened endoperiosteal and amidst callus at 3 d and 7 d in brain injury tibial fracture recovers rapidly and CGRP in the callus expresses highly after brain CGRP have significant effect on bone fracture healing，the expression of CGRP showed different expression in different period of fracture healing，CGRP increase in callous with brain injured fact indicated that CGRP were involved in fracture healing.