不同条件下保存样本对血清C_论文.docxVIP

不同条件下保存样本对血清C 作者：李向阳，徐丹，高旭红，李就鸿，祝胜郎，常巨平 【关键词】 C ［摘要］ 目的：观察在不同温度时间条件下保存血清样本对C反应蛋白(CRP)测定值的影响。方法：提取29例感染患者的血清标本，分装后放置在室温4 ℃和-20 ℃条件下；采用固相夹心胶体金法分别测定室温和4 ℃标本在1周、2周、3周、4周各时间点的CRP值，测定4周后-20 ℃冷藏标本的CRP值；将各测定值与血清分装后的即时检测值作比较，观察CRP的变化。结果：本组患者血清中含有中高浓度的CRP(±)mg/L，血清标本在-20 ℃条件下保存4周CRP能保持稳定，CRP检测值无明显变化；在室温或4 ℃条件下，中低浓度(CRP＜50 mg/L)标本能保持相对稳定1周～4周；高浓度标本(CRP≥50 mg/L)在1周～2周后CRP测定值出现一定程度升高，但升高程度不大(均值升高％～％)。结论：CRP是十分稳定的蛋白质分子，在室温或4 ℃保存血清标本1周～4周，其检测值仍具有参考价值；在-20 ℃条件下保存血清，可以保持CRP的稳定。 ［关键词］ C反应蛋白；测定；胶体金法 The Leverage of Different Storage Conditions on Creactive Protein Determination A Preliminary Study Abstract：Objective To observe the leverage of different storage conditions on Creactive protein The serum of 29 patients suffering infectious disease were obtained and storedatroom temperature,4 ℃ for 1 to 4 weeks，and -20 ℃ for 4 is determined with solid phase sandwich colloidal gold and 4 ℃ samples were measured after 1 week,2 weeks,3 weeks and 4 weeks，-20 ℃ samples after 4 those CRP values were compared with that of instantdetermined The patients had relatively medium to high CRP values of (±)mg/ could remain stable under -20℃ storage for 4 of medium CRP value(＜50 mg/L)could retain relatively samples with higher CRP(≥50 mg/L)concentration heightened,but the changes were not very large(median CRP increased by ～％ of original value).Conclusion CRP is a kind of protein molecule of pretty CRP determination is still valuable even if sample was stored at room temperature or 4 ℃ for 1 to 4 to maintain absolute stable，serum samples have to be kept under -20 ℃ condition. Key words：Creactive protein；Determination；Colloidal gold method 在感染、创伤、组织坏死、自身免疫性疾病等因素刺激下，C反应蛋白(Creactive protein,CRP)均可以作为可靠的全身或局部炎症反应指数；又因其测定方法简单、快速，使CRP成为临床中最为常用的急性期蛋白指标。本实验旨在观察在不同温度-时间条件下保存血清样本对CRP测定值的影响。 　　1　材料和方法 1．1　患者和标本采集　采集29例在我院住院患者的空腹或随机血标本。其中男性16例，女性13例，中位年龄26岁；分别罹患急性泌尿系统感染16例、呼吸道感染8例和其他感染性疾病5例。 1．2　血标本分装　使用8 ml真空干燥试管(无抗凝剂)采静脉血4 ml×1支，在室温下静置1 h~2 h，待血清析出，2 500 rmp×5 m