VEGF-CVEGFR-3与podoplanin在食管癌中的表达及其意义.docVIP

VEGF-C、VEGFR-3和podoplanin在食管癌中的表达及其意义 （作者:___________单位: ___________邮编: ___________） 【摘要】 目的 观察血管内皮生长因子C(VEGF-C)及其受体3(VEGFR-3)和肾小球上皮细胞整合膜蛋白(podoplanin)在食管癌组织中的表达，分析其与肿瘤淋巴结转移的关系。方法 免疫组织化学SP法检测76例食管癌组织中VEGF-C、VEGFR-3和podoplanin的表达及淋巴管密度(LVD)。结果 食管癌VEGF-C表达阳性率为63.1%，VEGF-C的表达与食管癌的淋巴结转移、TNM分期有统计学意义(P0.01, P0.05)，但与患者年龄、肿瘤大小及分化程度无关；癌旁组织淋巴管密度同淋巴结转移、VEGF-C阳性表达间有统计学意义(P0.01, P0.05)，podoplanin LVD值与VEGFR-3 LVD值相比，具有统计学意义(P0.01)。结论 食管癌组织中VEGF-C表达与肿瘤TNM分期、淋巴管密度和淋巴结转移有关，VEGF-C表达越高，淋巴管密度越大，淋巴结转移可能性越高。癌旁组织LVD同VEGF-C的高表达及淋巴结转移密切相关，食管癌组织中podoplanin标记的淋巴管密度较VEGFR-3标记的淋巴管密度更为精确，是一种更为满意的淋巴管内皮特异性标志物。 【关键词】 食管癌；淋巴结转移；血管内皮生长因子C；血管内皮生长因子受体-3；podoplanin 　　ABSTRACT: Objective To investigate the expression of vascular endothelial growth factorC (VEGF-C), its receptor3 (VEGFR-3) and podoplanin in tissue of esophageal cancer, and the relationship between their expression and tumor lymphatic metastasis. Methods Expression of VEGF-C, VEGFR-3 and podoplanin in 76 cases of ESC was detected by immunohistochemical SP method and lymphatic vessel density (LVD) was calculated. Results Positive immunostaining of VEGF-C in esophageal cancer tissue was 63.1%; the expression of VEGF- C was significantly associated with the depth of lymph node metastasis and tumor invasion (P0.01, P0.05, respectively), but not with the patient age, tumor size or differentiation. The average level of LVD was 15.39?.14, VEGFR-3 marked LVD was 9.27?.39, and podoplanin marked LVD was 22.09?.59. There were significant differences (P0.01). Conclusion There are clinical significance correlations VEGF-C expression, LVD expression and lymph node metastasis in esophageal cancer. The higher VEGF-C expression and LVD expression in esophageal cancer, the higher risk of lymph node metastasis in esophageal cancer; podoplanin marked LVD is more precise than VEGFR-3 marked LVD, and expression of podoplanin correlates closely with high expression of VEGF-C and LVD. It is a satisfactory lymphangial endothelium marker. 　　KEY WORDS