七叶皂苷钠对大鼠局灶性脑缺血再灌注后MMP9与LN表达的影响.docVIP

七叶皂苷钠对大鼠局灶性脑缺血再灌注后MMP9和LN表达的影响 （作者:___________单位: ___________邮编: ___________） 作者：张韶华，韩文江，李晓蕾，王秀丽 【摘要】 　　目的 研究七叶皂苷钠(sodium βaescin)对大鼠局灶性脑缺血再灌注后基质金属蛋白酶9(matrix metalloproteinase9, MMP9)和层黏连蛋白(laminin, LN)表达影响，为深入探讨七叶皂苷钠对脑的保护机制提供实验依据。方法 制备大鼠局灶性脑缺血再灌注模型，随机分为正常组、假手术组、缺血再灌注组和缺血再灌注七叶皂苷钠治疗组，后两组又分为缺血2h后再灌注3、6、12、24、72h和7d，共6个时间点，每时间点4只；用免疫组织化学法和图像分析观察其缺血区LN和MMP9的表达变化。结果 缺血再灌组MMP9在神经元的表达于再灌注6h开始增加，12h达高峰，24h又降低，7d最低，治疗组MMP9于再灌注同一时相有显著减少；LN在微血管的表达于再灌注12h明显减少，24h时达低峰，72h开始回升，治疗组在同一时相LN表达显著增多。结论 MMP9与LN可能参与脑血管源性脑水肿的形成发展，缺血再灌注早期七叶皂苷钠治疗通过减少MMP9的表达，增加LN的表达对缺血再灌注脑起保护作用。 【关键词】 七叶皂苷钠；基质金属蛋白酶9；层黏连蛋白；脑缺血再灌注；大鼠　 　　ABSTRACT: Objective To study the effect of sodium βaescin on the matrix metalloproteinase9 (MMP9) and laminin (LN) expression in rats after focal cerebral ischemia and reperfusion, and provide experimental evidence for the relationship of MMP9 and LN expression change with the neuroprotection of sodium βaescin. Methods The SpraqueDawley (SD) rat model of focal cerebral ischemia and reperfusion of cerebral middle artery was prepared. Fiftysix rats were randomly divided into the normal, sham, ischemia and reperfusion, as well as ischemia and reperfusiontreated with sodium βaescin groups. The latter two were further divided into six subgroups according to reperfusion time interval of 3h, 6h, 12h, 24h, 72h and 7d after brain ischemia, with 4 rats in each subgroup. Immunohistochemical staining and image analysis were used to measure the expression of MMP9 and LN in the ischemic region. Results The expression of MMP9 started to increase at 6h, reached the peak at 12h, decreased in 24h, and reached the lowest on 7d in cerebral ischemia and reperfusion group. The expression of MMP9 in the same time groups of the cerebral ischemia and reperfusiontreated with sodium βaescin group all obviously decreased (P0.05). The expression of LN in the microvessels started to decrease at 3h, reached the lowest at 24h, and increased at 72h in the cerebral ischemia