接种物胞外聚合物组分对颗粒污泥形成影响研究.docx

摘要在废水生物处理系统中，颗粒污泥代替絮状污泥可有减缓膜生物反应器的膜污染问题，EPS对颗粒污泥的形成有重要作用，但EPS的具体成份的影响尚未明晰，故研究如何快速生成颗粒污泥很有潜力。然而膜生物反应器中会产生颗粒污泥，本次研究提取来自膜生物反应中不同部位的污泥在摇床下进行污泥颗粒化培养，加入来自城市污水处理厂的普通污泥加以试验的对比。然后从显微观察、颗粒观察和粒径分布共同分析污泥颗粒化的规律模式，从而验证在摇床中是否有颗粒污泥形成，同时分析污泥中胞外聚合物的化学成分,从而找出影响颗粒污泥形成的最大相关性化学成分因子。关键词：好氧颗粒污泥、颗粒化、胞外聚合物、生物相AbstractIn the wastewater biological treatment system, the performance of the granular sludge mixture is better than that of the general activated sludge mixture. Therefore, it is promising to study how to quickly produce granular sludge. However, in the membrane bioreactor, granular sludge was produced. The sludge from different parts of the membrane bioreactor was extracted and the sludge was granulated under the shaker, and the comparison of the common sludge from the municipal sewage treatment plant was carried out. The Then, from the microscopic observation, particle observation and particle size distribution, the regular pattern of sludge granulation was analyzed to verify whether granular sludge was formed in the shaker and the chemical composition of the extracellular polymer in the sludge was also analyzed. So as to find the most relevant chemical composition factors that affect the formation of granular sludge.Keyword：Aerobic granular sludge、granulation、Extracellular Polymeric Substances、Biological phase目录1 绪论11.1研究背景11.1.1颗粒污泥11.1.2胞外聚合物21.2意义与内容21.2.1来源与创新点21.2.1目的与意义31.2.3技术路线流程图52 实验设计62.1内容与方法62.2实验不同来源样品性质分析72.2.1实验样品来源与处理72.2.2样品MLSS的测定82.2.3样品污泥生物相观察实验92.3污泥颗粒化培养与观察92.3.1污泥颗粒化实验102.3.2颗粒污泥的观察实验102.3.3颗粒粒度分布测定112.4样品胞外聚合物分析122.4.1胞外聚合物热提取132.4.2蛋白质测定实验142.4.3多糖测定实验152.4.4腐殖质测定实验152.4.5脂类测定实验162.4.5疏水性测定实验173 实验结果与讨论183.1实验样品性质分析结果183.1.1样品MLSS的测定结果183.1.2样品污泥镜检观察实验183.2颗粒污泥形成中观察结果203.2.1污泥颗粒化观观察结果与分析203.2.2污泥颗粒化过程中显微观察结果与分析223.2.3污泥粒径分布结果263.3样品胞外聚合物分析303.3.1蛋白质测定结果303.3.2多糖测定结果323.3.3腐殖质测定结果333.3.4脂类测定结果363.3.5疏水性分析结果363.3.6胞外聚合物成分比较分析374 结论38参考文献39致谢401绪论1.1研究背景1.1.1颗粒污泥颗粒污泥是微生物自凝聚形成的一种特殊形式的活性污泥，具有良好的沉降性能、密实的结构、较高浓度的生物量、较强的冲击负荷和抵抗有毒有害物质的能力【1】。污泥颗粒化是指废水生物处理系统中的微生物在适当的环