内皮细胞功能和心血管疾病.pptVIP

谢谢参与！ group 1, perfusion defect outside of the LAD distribution; group 2, no perfusion defect; group 3, perfusion defect within the LAD distribution. * Figure 1 Valsartan induces NO production in bovine aortic endothelial cells. Bovine aortic endothelial cells were treated with valsartan, 0–10 mM, for 24 h. Level of nitrite (A) or accumulation of intracellular cGMP (B) was measured by Griess and enzyme immunosorbent assay, respectively. The expression of endothelial NO synthase protein (C) was examined by western blot analysis. (D) Time course of NO production with 10 mM valsartan for 0–24 h. (E) DAF-2 DA fluorescence staining was performed to detect NO production in response to valsartan treatment (10 mM). (F) L-NAME (400 mM) pre-treatment abrogated the valsartan-induced nitrite accumulation. Data are mean+SEM from three independent experiments. *P , 0.05 vs. non-treated (A, B, and C) or vehicle-treated (D) group, #P , 0.05 vs. valsartan-treated group (F). * OMT * HT * 内皮细胞功能与心血管疾病 李 静 首都医科大学宣武医院 心脏科 内容 概述 心血管危险因素 心血管事件 治疗 血管内皮细胞的功能 血管壁和血管腔的机械、生物学屏障 调节血管张力 维持血管稳态 血管舒张 抗氧化 抗炎 抗增殖 抗凝 促进纤溶 Lerman A. Circulation. 2005 内皮功能不全 舒血管因子生物有效性下降 血管收缩物质作用增加 内皮依赖性血管舒张受损 保护能力下降 动脉粥样硬化最早期的功能改变 Michael EW, JACC, 2003 内皮功能检测方法 Andreas F. Circulation. 2012 EndoPAT 血管内皮功能 概述 心血管危险因素 心血管事件 治疗 传统心血管危险因素 Toggweiler S, et al. Clin Cardiol 2010 代谢性危险因素 Li J, et al. Mayo Clin Proc 2012 Muredach, Circulation, 2003 内皮功能与炎症 Fichtlscherer S et al. Circulation 2000 Li J, Circ J, 2012 内皮功能与冠状动脉钙化 Li J, et al. Circ J 2012 Balah, JACC CV imaging, 22009 Jone W, JACC, 2010 高血压导致内皮功能损害的机制 Kodlipet D, Curr Hypertens Rep. 2010 内皮功能不全反映了危险因素的危害程度 Bonetti PO, et al. Arterioscler Thromb Vasc Biol 2003 血管内皮功能 概述 心血管危险因素 心血管事件 治疗 内皮功能障碍心血管事件增加 Rubinshtein R et al. Eur Heart J 2010 冠心病患者内皮功能与预后密切相关 Sammy Y. Chan et al. JACC 2003 内皮功能状态与心肌缺血相关 Hasdai D, et al. Circul