Laboratory Glassware Austin Community College District实验室玻璃器皿斯汀社区学院区.pptVIP

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Laboratory Glassware Austin Community College District实验室玻璃器皿斯汀社区学院区.ppt

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Laboratory Glassware Austin Community College District实验室玻璃器皿斯汀社区学院区

Laboratory Glassware MLAB 1335 Immunology Serology Terry Kotrla, MS, MT(ASCP)BB Pipets (Pipettes) Laboratory instrument used to transport a measured volume of liquid. Three types of glass pipets used in the laboratory Volumetric Mohr Serological Mechanical Pipets Serological and Mohr pipets have different markings to assist in identifying them as they are used differently. Serological pipets are TD = to deliver. To accurately dispense the measured volume the last bit must be blown out. Mohr pipets are TC = to contain. These pipets are designed to dispense the correctly measured volume, so there will be a minute amount of liquid left in the tip. Markings TD pipets will have an etched or colored ring at the top of the pipet. TC pipets will have no rings although there may be a colored bar to indicate the volume. Volumetric Designed to transfer a fixed amount of liquid when filled to the mark, e.g. 10 mL and only 10 mL. There is generally only one fill-line on a volumetric pipet. For example a 5 mL volumetric pipette has one marking on it. This marking measures exactly 5 mL. of liquid, no more, no less.? Volumetric Designed to deliver a single volume precisely, the volume will be indicated near the top of the pipet, At the top of the pipet is an etched ring. Fluid must be drawn up the pipet to above the ring indicating the volume and then released slowly until the bottom of the meniscus is exactly at the ring. To transfer this volume to a second container, touch the pipette tip to the inside of the new container and allow the liquid to drain out. Serological - TD A pipet marked TD (the more common type of pipet) has been calibrated to deliver a specified volume of liquid. These pipets have no base mark, the graduations continue onto the tip and are graduated to deliver which means that ALL the measured liquid in the pipet must be delivered. A 5 mL. serological pipette with one-tenth milliliter graduations can be used to obtain any desired volume of liquid in