Leydig cell differentiation in mouse embryonic gonads在小鼠胚胎的性腺丸间质细胞的分化.docVIP

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Leydig cell differentiation in mouse embryonic gonads在小鼠胚胎的性腺丸间质细胞的分化.doc

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Leydig cell differentiation in mouse embryonic gonads在小鼠胚胎的性腺丸间质细胞的分化

Author’s institutions: 1Center for Animal Genomics, Veterinary Faculty, University of Ljubljana, Gerbi?eva 60, SI-1000 Ljubljana, Slovenia 2University of Zagreb, School of Medicine, Dept. Histology and Embryology, ?alata 3, 10000 Zagreb, Croatia 3Centre for Functional genomics and Bio-Chips, Institute of Biochemistry, Medical Faculty, University of Ljubljana, Zalo?ka 4, SI-1000 Ljubljana, Slovenia Title: Initiation of steroidogenesis precedes expression of cholesterolgenic enzymes in the fetal mouse testes Authors: 1Büdefeld T., 2Jezek D., 3Rozman D., and 1Majdic G. Short title: Steroidogenesis and cholesterol production in the fetal mouse testis Key words: Mouse, testis, fetal, human, 3beta-hydroxysteroid dehydrogenase, StAR, cyp51, NADPH cytochrome P450 reductase, immunocytochemistry Corresponding author: Gregor Majdic Center for Animal Genomics Veterinary Faculty, University of Ljubljana Gerbi?eva 60, SI-1000 Ljubljana Slovenia Phone: +386 1 4779210, Fax: +386 1 2832243 Email: gregor.majdic@vf.uni-lj.si Abstract Sexual differentiation is a carefully regulated process that ultimately results in a development of the male or female phenotype. Proper development of the male phenotype is dependent upon the action of testosterone, produced by Leydig cells, and antimullerian hormone, produced by Sertoli cells. Leydig cells start to produce testosterone around day 12.5 in the fetal mouse testis, and continue to produce high levels of testosterone throughout the gestation. In the present study we examined whether expression of two enzymes, lanosterol 14α-demethylase (cyp51) and cytochrome P450 NADPH reductase, involved in the cholesterol production, occurs simultaneously with proteins required for the production of steroid hormones. Immunocytochemical staining with antibodies against lanosterol 14α-demethylase , cytochrome P450 NADPH reductase, steroidogenic acute regulatory protein (StAR) and 3beta-hydroxysteroid dehydrogenase I (3β-HSD I) was used to determine the