人体胃内细菌数量相关素分析朱鸣,吴本俨,宫媛,徐雅萍.docVIP

人体胃内细菌数量相关因素分析 朱鸣，吴本俨，朱鸣，吴本俨，解放军总医院南楼消化科北京市解放军总医院南楼消化科. TelE-mail:benyanwu@ 解放军总医院南楼消化科解放军总医院南楼analysis of correlation factors of human gastric bacterial number ZHU Ming, WU Benyan, GONG Yuan, Xu Yaping Department of Gastroenterology, the General Air Force Hospital, Beijing 100036, China Correspondence: WU Ben-Yan, Department of Gastroenterology, South Building, the General Hospital of Chinese PLA, Beijing 100853, China [Abstract] AIM: To investigate the relationship of gastric bacterial number, gastric pH value, age, atrophic gastritis, Helicobacter pylori(Hp）infection and other factors. Methods: 69 objects, 52 male (45 objects≥60yr, 7 objects＜60yr), 1 female (10 objects≥60yr, 7 objects＜60yr). astric fluid and gastric tissue from gastroscope, and measured the pH value of gastric fluid. Aerobic and anaerobic bacteria culture of gastric tissue were performed, and then we counted the bacteria number after bacteria culture. Pathological examination of gastric tissue had been done and Hp infection had been detected. Results: Logistic regression indicated that the number of aerobic bacteria of gastric tissue related to the gastric pH value（P=0.032）, and was independent of sex, age, diabetes, Hp infection, and atrophic gastritis（P0.05）. The number of anaerobic bacteria of gastric tissue related to the gastric pH value（P=0.001）, diabetes（P=0.027）, and Hp infection（P=0.033）. Conclusion: The number of aerobic bacteria of human gastric tissue related to the gastric pH value, the number of anoxybiontic bacteria of gastric tissue related to the gastric pH value, diabetes, and Hp infection. [Key wards] stomach, gastric acid, bacterial overgrowth, Helicobacter pylori. 胃内细菌过度生长指的是细菌在胃内的非正常生长，是由于在正常生理状态下的特定的防御机制失效造成的[1]。正常人胃液的pH值为1.5～2.0，胃液里的盐酸可以杀死随食物进入胃内的细菌，因而对维持胃和小肠内的无菌状态具有重要意义[2]。在胃内pH值增大时，胃内细菌可能过度生长，可能导致细菌的感染机会增加。本实验通过对55例老年人及14例非老年人胃组织做细菌培养，初步探讨了人体胃内细菌数量与胃内pH值及其它因素的关系。 1 材料与方法 1.1 材料 仪器：电子胃镜（日本Olympus公司），内镜洗镜机（日本Olympus公司），Olympus BH-2 显微镜（日本Olympus公司），电热恒温培养箱（中国重庆