第二次微生物学实验.docxVIP

Continuous streak / Quadrant streak method[Principle]Isolation cultivationStreak a bacterial culture on a nutrient agar plate to separate the individual bacterial cells, each isolated cell will develop into a pure colony after incubation. There are two methods, including continuous streak method and quadrant streak dilution method.[Material]E.Coli Agar plate spirit-lamp inoculation oese[Procedure]1)Pick up bacteria: choose smooth inoculation rings and pick up a small amount of bacteria samples according to aseptic manipulation. 2) Divide the first area: the flat plate is inverted to the flame of the alcohol lamp, and the plate bottom is taken out with the left hand to make the flat surface roughly perpendicular to the desktop and let the flat plate face the flame. The right hand holds the inoculation ring containing bacteria. First, the 3~4 parallel lines are lightly delimit in the first area as the initial dilution bacteria source. Burn out the residual bacteria on the inoculation ring.In the rest area, the inoculation ring was cooled on the edge of the plate culture base, and the second area was transferred to the line position. The inoculation ring was moved to the second area through the first region (the source area), and then the 6~7 line was lightly divided in the second area.With the same operation, there are more parallel lines in the third area and the last zoning, and the lines in the last area are parallel to the first area (but not in line with the lines in the first area or second area! Finally, put the left-handed container bottom into the lid. Burn out the residual bacteria on the ring. 5, incubator of constant temperature3)The flat plate will be placed at 37 C for 2~3 days.[Result][Analysis]It can be seen clearly that individual bacterial cell was separated in the third region. The separation is successful. The separated bacterial cell is round, not transparent, white, big, flat and its surface is smooth, its margin is neat.Ultraviolet