pH响应的介孔二氧化硅纳米粒在药物运输及肿瘤治疗的应用研究.doc

HUNAN UNIVERSITY 毕业设计(论文) 设计论文题目： pH响应的介孔二氧化硅纳米在 药物运输及肿瘤治疗的应用 学生姓名： 邱鹏超 学生学号： 2 专业班级： 生物技术0802 学院名称： 生物学院 指导老师： 学院院长： 2012年5月28pH响应的介孔二氧化硅纳米颗粒在药物运输及肿瘤治疗的应用研究 摘 要 抗肿瘤药物一般都具有较大毒副作用，极大影响其在肿瘤治疗中的应用。如何提高药物的靶向性、减少用药量和降低运输过程中的毒副作用成为解决该问题的关键。随着纳米技术的发展，纳米材料在生物医学领域的应用也越来越广。介孔二氧化硅纳米颗粒具有较好的生物相容性、载药性、易于合成和修饰等特点，也受到广泛关注。本研究选择了具有较好生物相容性的聚丙烯酸（PAA）pH响应，药物运输，可控释放 Mesoporous Silica Nanoparticles Decorated with pH-Responsive Nanogated Ensemble for Drug Controlled Release and Tumor therapy Abstract Generally anticancer drugs have a great side effect, which limits their effectiveness in cancer therapy extremely. Thus there is a key question that how to improve the targeting capability of the drug reduce the dosage and cut down the toxic side effects during the transport process. As the development of the nanotechnology, there’s an increasing use of nanomaterials in the fields of biomedical. For their good biocompatibility, high drug loading efficiency and simplicity to be constructed and modified, Mesoporous Silica Nanoparticle (MSN) have drawn more and more interests. In our experiment, an acid-labile acetal molecule, 3,9-Bis (3-aminopropyl)-2,4,8,10-tetraoxaspiro[5.5] undecane(ATU) , was used as a linker between Poly(acrylic acid) (PAA)and carboxylated mesoporous silica nanoparticles to synthesize PAA grafted Mesoporous Silica Nanoparticles. The physical and chemical properties of the as-synthesized mesoporous silica nanoparticles were characterized by ZetaSizer analyses. DOX was chosen as a model molecule to assess its loading efficiency and controlled release behavior through fluorospectro photometer and confocal microscope. The results showed that the PAA grafted mesoporous silica nanoparticles was sensitive to pH. At neutral pH, as ATU keeps stable, PAA chains stay on the surface of the nanoparticles, leading to the closing of the mesopores. The DOX could hardly be released from the carriers. As the pH value decreased, the stability of ATU reduced, and mo