与杂质的量、试样的pH及离子强度酶的物化特性大小、pI.pptVIP

7.在纯化过程中酶活力的损失主要原因：蛋白质变性 避免方法：改变温度和pH 其他原因：辅助因子被去除 酶分离纯化的方案设计 a. Sketch the elution profile expected if this mixture is run on a Sephadex G-100 gel filtration column run in 50 mM phosphate buffer, pH 6. Label the peaks. b. Sketch the gel obtained from running these proteins on an SDS polyacrylamide electrophoresis gel at pH 8.0, (after staining and destaining). c. What predictions can you make about the results of a native PAGE at pH 7.6 ( state any assumptions you might need to make about the % acrylamide in the gel ) d. Sketch the elution profile of these proteins from a carboxyme