不同类型佐剂或联合抗原 对balbc小鼠免疫系统的作用-effects of different adjuvant or combined antigens on the immune system of balbc mice.docx

目录缩略词一览表i摘 要ivAbstractvi第一章 引言 ....................................................................................................................... 11 选题背景 ................................................................................................................. 12 研究目的及意义 ..................................................................................................... 33 研究内容 ................................................................................................................. 3第二章 BALB/c 小鼠肌肉注射铝佐剂、MF59 对免疫系统作用的研究61 实验材料和方法 ..................................................................................................... 61.1 受试品和对照品 .......................................................................................... 61.2 主要试剂和仪器 .......................................................................................... 61.3 溶液配制 ...................................................................................................... 81.4 实验动物的饲养和管理 .............................................................................. 91.5 检测和分析方法 ........................................................................................ 101.6 数据处理方法 ............................................................................................ 152 实验结果 ............................................................................................................... 162.1 免疫系统非功能性评价 ............................................................................ 162.2T 细胞依赖性抗体反应（TDAR）192.3 脾细胞各细胞表型分析 ............................................................................ 192.4 外周血细胞因子检测 ................................................................................ 232.5 基因表达谱分析 ........................................................................................ 243 讨论 ....................................................................................................................... 293.1 佐剂选择依据 ..............................