吡格列酮对正常mc3t3-e1成骨细胞增殖 凋亡以及功能蛋白表达影响-effect of pioglitazone on proliferation, apoptosis and expression of functional protein in normal mc 3t3 - e1 osteoblasts.docx

concentrationabove5μmol/L(P0.05);ExpressionsofALPandBMP-2wereincreasingwiththetimefrom24to48h(P0.05),butnotstatisticallysignificantofOCN.Conclusions：pioglitazonehasabiphasiceffectonmousepreosteoblasticcellsMC3T3-E1exposedtonormalglucose:inacertainconcentration(5μmol/L),itcaninhibitcellapoptosis、promotecellproliferationandexpressionsoffunctionalprotein;whileacceleratecelldeath、suppresscellproliferationandfunctionobviouslyathigherconcentrations(≥10μmol/L);underthenormalglucose,pioglitazonecannotonlyactivatePPARγandreducebone-relatedsignalingpathways,leadingtodecreasedactivityofosteoblasts;butalsoactivateotherpathwaystoantagonizetheroleofPPARγ-inducedboneinjury;normalosteoblastsmayhavetheself-regulatorymechanism,low-dosepioglitazoneisabletostartaprotectionalmechanismonosteoblasts.Keywords：pioglitazone;MC3T3-E1osteoblasticcell;normalglucose;proliferation;apoptosis;functionalprotein;peroxisomeproliferator-activatedreceptorgamma;osteogenictranscriptionfactor(Runx2)英文缩略词表ABBREVIATIONS英文缩写英文名称中文名称ADAAmericanDiabetesAssociation美国糖尿病协会LDMEMlowglucosedulbeccosmodifiedeaglesmedium低糖改良伊格(氏)培养基DMSOdimethylsulfoxide二甲基亚砜FBSfetalbovineserum胎牛血清PBSphosphatebufferedsodium磷酸盐缓冲液PPARγperoxisomeproliferator-activatedreceptorgamma过氧化物酶增殖物活化受体γTZDsthiazolidinyldiones噻唑烷二酮类PIOpioglitazone吡格列酮SPSSstatisticalpackageforscoialscience社会科学统计学软件ODopticaldensity光密度值IODintegratedoptiondensity累积光密度值OPosteoporosis骨质疏松IGRimairedglucoseregulation糖调节受损MAPKmitogenactivatedproteinkinase丝裂原活化蛋白激酶Runx2Runtrelatedgene2runt域基因家族2BMP-2bonemorphogeneticprotein-2骨形态发生蛋白-2OCNosteocalcin骨钙素ALPalkalinephosphase碱性磷酸酶Elisaenzyme-linkedimmunosorbentassay酶联免疫吸附法RIAradioimmunoassay放射免疫分析法FCMflowcytometer流式细胞术RT-PCRreversetranscriptionpolymerasechainreaction逆转录聚合酶链式反应学位论文原创性声明和版权使用授权书学位论文原创性声明本人郑重声明：所呈交的学位论文，是本人在导师的指导下，独立进行研究工作所取得的真实成果。除文中已注明引用的内容外，本论文不含任何其他个人或集体已经发表或撰写过的作品成果。对本论文的研究做出重要贡献的个人和集体，均已在文中以明确方式标明。本人完全意识到本声明的法律结果由本人承担。作者签名（手写）：导师签名（手写）：年月日年月日学位论文版权使用授权书本学位论文作者完全了解学校有关保留、使用学位论文的规定，同意学校保留并向国家有关部门或机构（如国家图书馆、中国学术期