YC-1预处理对小鼠肠缺血再灌注所致肺损伤时TLR4mRNA表达的影响.DOCVIP

基金项目：上海市自然科学基金(12ZR1417200)作者单位：200011，上海交通大学医学院附属第九人民医院麻醉科（陈超、徐睿、李启芳、姜虹）MyD88对小鼠肠缺血再灌注致急性肺损伤的影响陈超，徐睿, 李启芳，姜虹【摘要】目的 观察髓样分化因子88（MyD88文中首次出现的英文缩写都应同时有中文解释。）对小鼠肠缺血再灌注致急性肺损伤的影响。方法 6~8周龄雄性MyD88基因敲除小鼠(MyD88-/-)及其野生型C57BL/6小鼠各24只，两组品系小鼠组内随机分为2组(n=12)：假手术组（S组）及肠缺血再灌注组（I/R组）。麻醉后制备肠缺血再灌注致急性肺损伤模型。处死小鼠后开胸取肺标本， H-E染色后在显微镜下观察肺组织病理学改变，计算肺湿干重比，ELISA检测肺组织TNF-α和IL-1β表达，RT-PCR检测TLR4mRNA表达水平。结果 与C57BL/6小鼠I/R组比较此处应该是野生型C57BL/6小鼠的I/R组吧， MyD88-/-小鼠I/R组肺组织病理学损伤程度明显减轻，肺湿干重比、肺血管渗透性降低（P0．05）；并下调肺组织TLR4 mRNA，TNF-α和IL-1β (与C57BL/6小鼠I/R组比较同d2P0.05)。结论 MyD88基因敲除可明显减轻小鼠肠缺血再灌注后肺损伤,减轻肺水肿及肺血管通透性,下调肺组织TLR4 mRNA，TNF-α和IL-1β。文中首次出现的英文缩写都应同时有中文解释。此处应该是野生型C57BL/6小鼠的I/R组吧同d2【关键词】髓样分化因子88；缺血再灌注；肺损伤；Toll样受体4The effect of MyD88 in the acute lung injury mice of which induced by Intestinal-ischemia reperfusion. 【Abstract】请做相应修改Objective To investigate the effect of myeloid differentiation factor 88 on acute lung injury(ALI) induced by intestinal ischemia-reperfusion(I／R) injury in mice． Methods Twenty-four healthy male C57BL/6 mice were randomly divided into 2 groups(n=12 each)：Sham operation group(group S)；intestinal I／R group(group I/R)；Twenty-four healthy male MyD88-/- mice were randomly divided into 2 groups(n=12 each)：Sham operation group(group S)；intestinal I／R group(group I/R).Intestinal I／R injury was induced by clamping the superior mesenteric artery for 45min and the mice were sacrificed at 6 h of reperfusion.The lungs were immediately moved for microscopic examination,determination of W/D lung weight radio, levels of TNF-α and L-1β（by ELISA）and TLR4 mRNA expression in the lung tissue(by RT-PCR)．Results Microscopic examination showed that there were collapse or consolidation alveoli,edema and infiltration of neutrophils in the lung parenchyma in C57BL/6 I/R group.Intestinal ischemia reperfusion significantly increased W／D lung weight ratio, levels of TNF-α and L-1β, and TLR4 mRNA in the lung tissu．The lung injury was significantly ameliorated in group MyD88-/- I/R as compared to group C57BL/6 I/R. M