猪肠道酵母菌分离及鉴定.docxVIP

猪肠道酵母菌的分离与鉴定摘要：采用选择性培养基，从健康成年猪空肠分离到4株野生型酵母菌株，编号为:JSY1,JS Y2, JSY3, JSY4，通过形态学和生理生化特征对其进行了属的鉴定。并进一步用PCR方法扩增了这些菌株比较保守的25SrDNA片段，通过对其序列分析，构建系统树，更加准确地确定了这些菌株的遗传背景及种属关系，为转基因微生态制剂的制备奠定了良好的基础。关键词：酵母菌；分离鉴定；猪肠道；25SrDNAIsolation and Identification of Yeasts from Intestines of SwineZHou Ping ,WANG He-ping, ZHENG Qing-wei(1.Bengbu Medical College, AnhuiBengbu233030;Inner Mongolia Agricultural University, Hohhot 010018)Abstract: The four wild yeasts were isolated from jejunum of healthy and grown-up swines with selective medium,coded JSY1,JSY2,JSY3,JSY4.The preliminary generic identification was done thro