纳米tio2和重金属铅对环境微生物e.coli联合毒性作用的评价分析-evaluation and analysis of joint toxicity of nano tio 2 and heavy metal lead to environmental microorganism e coli.docxVIP

华中科技大学硕士学位论文 华 中 科 技 大 学 硕 士 学 位 论 文 5 5 and OD600 is studied. The combined toxic effects of nano-TiO2 and PbAc on E. coli growth, antioxidant enzymes, proteins, genetic material DNA are explored in 4 × 4 full factorial design, and their interactions are identified using response surface analysis. Results: SEM results show that compared with single nano TiO2, adding PbAc induces the aggregation and surface property changes of TiO2 nanoparticles. DLS results show that nano-TiO2 particle size is significantly increased with decreased concentration of nano-TiO2 or combined with an increasing dose of PbAc (P 0.05). Flame atomic absorption results show that nano-TiO2 adsorbs Pb2+ iron, and its adsorption rates are significantly increased with increasing concentration of nano-TiO2(P 0.05). The relationship between OD600 and E. coli concentration is not linear, but curved; a mathematical model is fitted and checked. The results prove that the model has a high accuracy and compared with the plate counted numbers its error is within ±3%. Growth curves of E.coli showed that nano-TiO2 and PbAc inhibit its growth, and the combined mixtures exhibit severer inhibitions. Full factorial analysis showed that nano-TiO2 and PbAc both have adverse effects on E. coli growth, antioxidant enzymes, protein carbonyl levels, DNA oxidation levels; and in their mixtures, compared with the corresponding effect of single substance, the combined toxicity is not significant at low doses of nano TiO2 or PbAc. Response surface analysis showed that there is a significant interaction between nano TiO2 and PbAc, it is identified as antagonism. Conclusion: Nano TiO2 and PbAc significantly influence each other: nano TiO2 adsorbs PbAc, reducing its effective concentrations; PbAc induces the aggregation and surface property changes of nano TiO2. Nano-TiO2 and PbAc have toxic effects on E. coli growth, antioxidant enzymes, proteins, DNA, while the combined effects are less than