诺贝尔生理医学奖让基因沉默的秘密RNAi ppt课件.pptVIP

RNA interference  -gene silencing by double-stranded RNA;歷史年表;;Andrew Z. Fire ;;Injection of unc-22 RNA into the gonad of C. elegans Injection of mex-3 RNA into C. elegans Sense RNA Anti sense RNA ;; Injection of mex-3 RNA into C. elegans;;Process of RNA interference;;Drosha;Pasha;;Dicer;;;　將dsRNA切割成21-25nt的siRNA ;RISC;;RNAi擴增系統;Short hairpin RNA(shRNA);;Functional genomics;Survivin keeps the integrity of microtubule so it is needed in cell division ;The hope of HIV therapy; Knockdown in gp41 or gp120 Advantage: that would not affect the normal performance of human’s immune system.