UNR调控上皮性卵巢癌细胞上皮间质转化的作用.docVIP

UNR调控上皮性卵巢癌细胞上皮间质转化的作用 　　[摘要] 目的 ?z测UNR基因在上皮性卵巢癌细胞中的表达，并观察其对卵巢癌细胞上皮间质转化（EMT）的影响。 方法 采用实时荧光定量PCR（qPCR）检测并比较体外培养的人上皮性卵巢癌细胞系SKOV-3、HO-8910、ES-2和永生化人卵巢上皮细胞系MOODY中UNR基因的表达，采用qPCR和Western blot检测卵巢癌SKOV-3细胞的UNR-RNAi干扰效率，应用Transwell侵袭小室实验检测SKOV-3细胞的侵袭能力，采用Western blot检测SKOV-3细胞E-cadherin的蛋白表达。 结果 UNR在上皮性卵巢癌细胞系SKOV-3、HO-8910、ES-2中均有显著表达。shUNR转染SKOV-3后，Transwell侵袭小室实验显示，UNR-RNAi组穿膜细胞数为每视野（29.33±5.14）个，显著低于阴性对照组（76.00±5.42）个（P 0.01）。此外，Western blot显示UNR基因干扰后SKOV-3细胞中E-cadherin的蛋白表达显著上调。 结论 shUNR可以抑制卵巢癌SKOV-3细胞UNR基因的表达，降低卵巢癌细胞的侵袭能力。干扰后E-cadherin的表达上调提示UNR可能促进卵巢癌EMT的发生，以UNR基因为靶点的干扰技术有望成为卵巢癌治疗的新策略。 　　[关键词] RNA干扰；卵巢癌；UNR；上皮间质转化 　　[中图分类号] R737.3 [文献标识码] A [文章编号] 1673-7210（2017）10（c）-0021-05 　　Role of UNR in regulating the epithelial mesenchymal transition of epithelial ovarian cancer cells 　　WENG Jianrong MIAO Yi SUN Yunyan 　　Department of Gynecology and Obstetrics， the First People′s Hospital Affiliated to Shanghai Jiao Tong University， Shanghai 200080， China 　　[Abstract] Objective To detect the expression of UNR gene in epithelial ovarian cancer cells， and to observe the effect of UNR gene on epithelial mesenchymal transition （EMT） of ovarian cancer cells. Methods Real-time quantitative PCR （qPCR） was used to detect and compare the expression of UNR gene in human epithelial ovarian cancer cell lines SKOV-3， HO-8910， ES-2 and immortalized human ovarian epithelial cell lines MOODY in vitro. qPCR and Western blot were used to detect the UNR-RNAi interfering efficiency of ovarian cancer cell SKOV-3， the invasion ability of SKOV-3 cells was detected by Transwell invasion assay. The protein expression of E-cadherin in SKOV-3 cells was detected by Western blot. Results UNR was significantly expressed in the epithelial ovarian cancer cell lines SKOV-3， HO-8910 and ES-2. After SKOV-3 was transfected by shUNR， Transwell assay showed that the number of transmembrane cells in UNR-RNAi group was （29.33±5.14） per field， which was significantly lower than that in negative control group