肺结核患者th1th2tregth17免疫应答临床研究.docVIP

肺结核患者Th1Th2TregTh17免疫应答的临床研究 　　[摘要] 目的 研究肺?Y核患者Th1/Th2/Treg/Th17免疫应答情况。 方法 选择2013年5月～2016年10月在解放军第四五八医院接受治疗的复治肺结核和初治肺结核患者，分别作为复治结核组（n=38）和初治结核组（n=47）；另取同期体检的健康志愿者作为对照组（n=50）。检测外周血中Th1、Th2、Treg、Th17细胞及相应细胞因子含量，血清中炎症介质的含量。 结果 复治结核组和初治结核组外周血中Th1、Th17及IFN-γ含量均显著低于对照组，Th2、Treg及IL-4、IL-5、IL-10、IL-17、TGF-β、HMGB1、sTREM1、MCP1、HBD2含量均显著高于对照组（P 0.05）；复治结核组外周血中Th1、Th17及IFN-γ含量均显著低于初治结核组，Th2、Treg及IL-4、IL-5、IL-10、IL-17、TGF-β、HMGB1、sTREM1、MCP1、HBD2含量均显著高于初治结核组（P 0.05）；HMGB1、sTREM1、MCP1、HBD2与Th1、Th17呈负相关，与Th2、Treg呈正相关（P 0.05）。 结论 肺结核患者病情变化与Th1/Th2/Treg/Th17免疫应答失衡存在密切关联。 　　[关键词] 肺结核；免疫应答；辅助性T细胞；炎症介质 　　[中图分类号] R521 [文献标识码] A [文章编号] 1673-7210（2017）09（b）-0109-04 　　Clinical study of Th1/Th2/Treg/Th17 immune response in patients with tuberculosis 　　CHENG Tao1 WU Weiling2 HUANG He3 　　1.Department of Infectious Disease， 458th Hospital of PLA， Guangdong Province， Guangzhou 510602， China； 2.Department of Respiratory Medicine， 458th Hospital of PLA， Guangdong Province， Guangzhou 510602， China； 3.Guangdong Academic Exchange Center of Medical Sciences Guangdong Medical Information Institute， Guangdong Province， Guangzhou 510180， China 　　[Abstract] Objective To study Th1/Th2/Treg/Th17 immune response in patients with tuberculosis. Methods Patients with recurrent tuberculosis and primary tuberculosis who received treatment in 458th Hospital of PLA during May 2013 to October 2016 were selected as recurrent tuberculosis group （n=38） and primary tuberculosis group （n=47） respectively； healthy volunteers who received physical examination during the same period were selected as control group （n=50）. The contents of Th1， Th2， Treg and Th17 cells as well as corresponding cytokines in peripheral blood， and the contents of inflammatory mediators in serum were determined. Results Th1 and Th17 cell contents as well as IFN-γ contents inperipheral blood of recurrent tuberculosis group and primary tuberculosis group were significantly lower than those of control group while Th2 and Treg cell content