纸质文物上一株交链孢霉生物学鉴定和其培养条件的优化.pdfVIP

Bioprocess 生物过程, 2016, 6(1), 24-34 Published Online March 2016 in Hans. /journal/bp /10.12677/bp.2016.61004 The Biological Identification and Optimization of Culture Condition for a Alternaria Strain Mold on Paper Relics 1 2 1 3 1 1* Yuwei Zhong , Bo Liu , Dacheng Duan , Chenshu Liu , Qijiu Li , Songbin Ben 1 Liaoning University of Life Sciences, Shenyang Liaoning 2 Liaoning Provincial Museum, Shenyang Liaoning 3 Liaoning Province Shiyan High School, Shenyang Liaoning th th rd Received: Feb. 26 , 2016; accepted: Mar. 18 , 2016; published: Mar. 23 , 2016 Copyright © 2016 by authors and Hans Publishers Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). /licenses/by/4.0/ Abstract Objective: To isolate and identify the fungus on paper heritage as well as optimized culture condi- tions. Method: Through morphology (three implantation, microscopic observation microstruc- ture) and Molecular Biology 18S rDNA ITS sequence amplification a strain on the paper relics is identified. The fungal culture condition is optimized by using single factor analysis and orthogonal test. Results: Two identified results showed that it was a strain of Alternaria mold (Alternaria eichhorniae). The optimum for its cultivation conditions is 28˚C, pH 5.5, shaking frequency 125 r/min. Conclusion: The strain is identified as a Alternaria mold (Alternaria eichhorniae) whose culture condition is easier to achieve in our laboratory, which makes basis for further analysis of components of mildew