褪黑素对海人酸致痫大鼠海马内tgf-β3水平的影响-effect of melatonin on tgf - β 3 level in hippocampus of epileptic rats induced by kainic acid.docxVIP

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褪黑素对海人酸致痫大鼠海马内tgf-β3水平的影响-effect of melatonin on tgf - β 3 level in hippocampus of epileptic rats induced by kainic acid.docx

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褪黑素对海人酸致痫大鼠海马内tgf-β3水平的影响-effect of melatonin on tgf - β 3 level in hippocampus of epileptic rats induced by kainic acid

华华中科技大学硕士学位论文 5 5 Most scholars have attached importance to antiepileptic action of melatonin. Our laboratory has found MT could inhibit the epilepsy induced by glutamic aid and coriamyrtin, which may act by reduing the expressions of NR1 and Glu, inhancing the GABA and GABRA1, downregulating the level of cAMP, cGMP and upregulating 5-HT. Although the antiepileptic effect of melatonin has been reported in a number of reports, little is known about the mechanisms underlined its antiepileptic action and the role of TGF-β3 in the epileptogenesis. The objectives of our present work were to study melatonins antiepileptic properties on kainic acid-induced epilepsy, and to probe the mechanisms of melatonins antiepileptic role by observing the changes of TGF-β3 level and the protective action on neurons in hippocampus. In our study, the changes of TGF-β3 in the hippocampus of epileptic rats were detected by immunohistochemistry staining method SABC and RT-PCR on the level of cell and gene respectively. The observation of rats’ behavior showed that no seizure activity occured in the NS group，but III-V grade of seizure can be seen in the KA group and 0-III grade in the MT+KA group. Immunohistochemistry staining indicated that TGF-β3 expressed in hippocampus of all groups, we can see immunoreactive neurons in pyramidal cell layer of hippocampus CA1－CA3 and granule cell layer of dentate gyrus in each group. The expression in the KA and MT+KA group is higher than that in the NS group, moreover MT+KA group is higher than KA group, the differences between each group are significantly( P 0.05). The results of RT-PCR showed the same tendency of changes on the level of TGF-β3 mRNA as it described in the immunohistochemistry. The morphology of TGF-β3 change indicated by immunostaining is coincident with the relative content of mRNA change in hippocampus detected by RT-PCR. Pretreatment with rat, the number of TGF-β3 positive neurons and the relative contents of TGF-