木质纤维素乙醇发酵及生物炼制课件.pptVIP

纤维素酶最适pH定向进化流程图 基因水平改造2 pH对纤维素酶EG II突变体酶活性的影响 Effect of Recombinant Expression 1.3X Celluclast + A 1.00 0.76 Celluclast +A + B PCS hydrolsysis, 50 g-scale, 120 hr, 50 °C 基因水平改造3 Identification of proteins secreted by T. reesei Swollenin EG II EG III Xyn I EG I Bgl II CBH II EG V CBH I EG II ? ? ? EG IV Cel74 UC Bgl I 蛋白质水平研究 Improvement of PCS-hydrolyzing cellulases by BG addition 发酵工程研究 Other fungal proteins as Celluclast-booster ~2X reduction in enzyme loading Fungus Z, 1X Tr + Z (1:1), 0.5X Tr, 1X 6X Significant biochemical improvementsin cellulase efficiency has been made over t