ZEB1ZEB2及miR―200a在上皮性卵巢癌I期患者中表达及临床意义.docVIP

ZEB1ZEB2及miR―200a在上皮性卵巢癌I期患者中表达及临床意义 　　摘 要：目的：观察ZEB1、ZEB2及miR-200a在上皮性卵巢癌I期患者中的表达，分析其表达水平与组织学的关系。方法：收集120例上皮性卵巢癌I期患者作为病例组，120例卵巢上皮性良性肿瘤患者作为对照组，120例因子宫因素切除子宫及双附件的患者作为空白组，观察ZEB1、ZEB2及miR-200a在不同组的表达。同时研究在上皮性卵巢癌I期患者的不同组织学分型中以上指标的表达特点。结果：病例组中ZEB1阳性率显著高于对照组和空白组（P0.5），病例组miR-200a较对照组、空白组显著上调（P0.5）。miR-200a在透明细胞癌的上调率显著高于粘液性囊腺癌、浆液性囊腺癌、子宫内膜癌（P0.05）。结论：本研究提示在早期上皮性卵巢癌，miR-200a可能作为诊断指标；尤其对于透明细胞癌敏感度较高。而ZEB家族因其表达的时段性，在临床上的应用仍有待发掘。 　　关键词：ZEB家族，miR-200家族，上皮性卵巢癌 　　Abstract： Objective： To observe expressions of ZEB1， ZEB2 and miR-200a in epithelial ovarian cancer patients of phase I， and analyze their characteristics in histology. Methods： Set 120 cases of epithelial ovarian cancer of Phase I as the case group， 120 cases of patients with epithelial ovarian benign tumor as a control group， and 120 cases of patients accepted oophorectomy due to uterine factors as a blank group. Towards the study we supposed to obverse expressions of ZEB1， ZEB2 and miR -200a in different groups，also to study the characteristics of expressions in different histological type. Results： The positive rate of ZEB1 in case group significantly higer than others（P 0.5）.The rate of miR-200a up-regulation in case group was significantly higher （P 0.5）compared with the control group and blank group .The rate of miR-200a up-regulation in clear cell carcinoma was significantly higher than mucinous adenocarcinoma， serous adenocarcinoma and endometrial cancer （P 0.05）. Conclusion： This study suggests that in the early epithelial ovarian cancer， miR-200a may be used as a diagnostic indicator，especially for clear cell carcinoma. The expressions of ZEB family was changing in different stage，so the clinical application remains to be discovered. 　　Keywords： ZEB family， miR-200 family， epithelial ovarian cancer 　　卵巢恶性上皮肿瘤是死亡率最高的妇科恶性肿瘤。因卵巢的解剖特点，早期病变不易发现，大部分患者经诊断已属晚期。上皮-间质转化（epithelial-mesenchymal transition，EMT）在卵巢恶性上皮肿瘤的发生和侵袭转移的进程中起关键作用，此外EMT还与食管癌、口腔癌、乳腺癌、鼻咽癌、直肠癌等上皮性来源的恶性肿瘤的原位浸润和远处转移有关[1]。EMT的发生由一系列蛋白、信号通路、转录因子、生长因子等调