三种疱疹科病毒潜伏感染与肺癌关系研究.docVIP

三种疱疹科病毒潜伏感染与肺癌的关系研究 　　[摘要] 目的:探讨三种疱疹科病毒与肺癌的关系。方法:取肺癌、癌旁正常组织和非特异性炎症肺组织石蜡包埋,应用PCR方法测定EB病毒(EBV)、巨细胞病毒(CMV)和人类疱疹病毒6型(HHV-6)DNA,分析三种疱疹科病毒与肺癌的关系。结果:PCR检测EBV、CMV、HHV-6结果,肺癌组织分别为58.4%、33.3%、38.9%,癌旁组织为45.5%、36.4%、30.3%,非特异性炎症肺组织为36.4%、18.2%、27.3%。同癌旁组织比较,肺癌组织中EBV具有较高的感染率(P0.05);同炎症组织比较,三种病毒均有较高的感染率(P0.01),癌旁组织与炎症组织比较,三种病毒亦有较高的感染率(P0.01)。结论:EBV感染与肺癌的发生有关,肺癌组织中EBV感染率明显高于癌旁组织及非特异性炎症肺组织。 　　[关键词] 肺癌;EB病毒;巨细胞病毒;人类疱疹病毒6型 　　[中图分类号] R563 [文献标识码] B [文章编号] 1673-7210(2010)04(c)-104-02 　　 　　The relationship of three kinds of herpersvirus and lung carcinoma 　　LI Xiaodong1, FENG Xuewei2 　　(1.Department of Emergency Medicine, the Affiliated Fengtian Hospital of Shenyang Medical College, Shenyang110024, China; 2.Department of Respiratory, Shengjing Hospital of China Medical University, Shenyang 110003, China) 　　[Abstract] Objective: To explore the relationship between three kinds of herpersvirus and lung carcinoma. Methods: Paraffin-embedded pulmonary carcinoma, normal paracarcinoma tissue and nonspecific inflammation tissues were used to detect the DNA of Epstein-Barr virus, cytomegalovirus and HHV-6 by polymerase chain reaction (PCR). And the relationship of three kinds of herpes virus and lung cancer was analyzed. Results: The positive rate of EBV, CMV and HHV-6 in carcinoma was 58.4%, 33.3%, 38.9%, in paracarcinoma was 45.5%, 36.4%, 30.3%, in nonspecific inflammation tissues was 36.4%, 18.2%, 27.3% respectively. Compared to paracarcinoma tissue EBV in the pulmonary carcinoma tissue had more infection rate (P0.05); compared to inflammation tissues, there was higher infection rate in three kinds of virus (P0.01); compared to inflammation tissues, there was higher virus infection rate of three kinds of virus in paracarcinoma tissue (P0.01). Conclusion: There is a relationship between EB virus infection and pulmonary carcinoma, the EBV infection in pulmonary carcinoma is higher than those in the normal paracarcinoma tissue and nonspecific inflammation tissues significantly. 　　[Key