儿童急性淋巴细胞白血病患者细胞因子IL―2和IL―10水平变化临床研究.docVIP

儿童急性淋巴细胞白血病患者细胞因子IL―2和IL―10水平变化临床研究 　　摘要：目的 探讨儿童急性淋巴细胞白血病（ALL）患者初步诊断时和化疗完全缓解（CR）后血清中IL-2、IL-10水平的变化。方法 收集确诊为ALL且经过化疗获得完全缓解的患儿30例及健康儿童20例，采用流式细胞术（FCM）测量初诊组、完全缓解组（CR）、健康对照组血清中IL-2、IL-10的水平，比较化疗前后这两种细胞因子含量的变化，并分别与健康对照组做对比。结果 初诊组患儿IL-2水平（ 3.59±1.18）pg/ml与正常对照组儿童IL-2水平（6.22±2.03）pg/ml相比较明显下降（ P0.001），初诊组IL-10的水平（15.88±4.73）pg/ml与正常儿童IL-10的水平（ 5.69±3.44 ）pg/ml相比较则显著升高（P0.001）。随着化疗的进行IL-2水平（ 4.78±1.57 ）pg/ml明显上升，（P=0.005），IL-10水平（ 8.14±2.82 ）pg/ml显著下降（ P0.001），都未达到正常儿童水平（P= 0.034和P=0.036）。但是差异均有统计学意义（P0.05）。结论 细胞因子IL-2和IL-10可能在儿童ALL的发生、发展过程中发挥了免疫调节功能，其含量的变化在一定程度上能够反应儿童ALL患儿体内的免疫功能状态的好坏。 　　关键词：儿童；急性淋巴细胞白血病；IL-2IL-10；流式细胞术 　　Abstract：Objective To investigate the children with acute lymphoblastic leukemia（ALL）patients at initial diagnosis and chemotherapy complete remission（CR）after IL-2 in serum，the change of IL-10 level.Methods Diagnosed with ALL after chemotherapy and 20 cases of complete remission in 30 cases of children and healthy children by flow cytometry（FCM）measurement diagnosis group，complete remission group（CR），healthy control group serum IL-2，IL-10 level changes of these two cytokines were compared before and after chemotherapy，and compared with the healthy control group respectively.Results The level of IL-2（3.59±1.18） pg/ml in the newly diagnosed group was significantly lower than that in the normal control group （6.22±2.03） pg/ml（P0.001）.The level of IL-10 in the first visit group（15.88±4.73）pg/ml was significantly higher than that of the normal children （5.69±3.44），IL-10（P0.001），compared with the normal children.With chemotherapy were IL-2 level （4.78±1.57）pg/ml significantly increased（P=0.005），IL-10（8.14±2.82）pg/ml decreased significantly（P0.001），has not reached the level of normal children（P= 0.034 and P=0.036）.But the differences were statistically significant（P0.05）.Conclusion The cytokines IL-2 and IL-10 may play an immunoregulatory function in the development and progression of ALL in children.The changes of their contents can reflect the immune fun