棉秆沼气发酵生物预处理及接种物的驯化s7mjo.docxVIP

微生物学通报 APR 20, 2010, 37(4): 513?519 Microbiology China ? 2010 by Institute of Microbiology, CAS HYPERLINK mailto:tongbao@ tongbao@ 研究报告 研究报告 棉秆沼气发酵生物预处理及接种物的驯化 白云 李为* 陈春 廖鹏 (华中科技大学生命科学与技术学院资源生物学与生物技术研究所 湖北 武汉 430074) 摘 要: 通过多代淘汰的方法, 筛选了一组稳定高效的棉秆降解复合菌系-MEG 复合菌系。将该菌 系接入棉秆, 静置处理 7 d 后进行沼气发酵, 其产气量较未经预处理的提高了 25%。变性梯度凝胶 电泳(DGGE)分析结果表明, 生物预处理后棉秆沼气发酵系统的细菌群落结构多样性较未经预处 理的丰富。同时, 比较了采用 6 种驯化方法获得的接种物对棉秆沼气发酵日产气量和累积产气量 的影响, 结果表明, 采用河底泥、臭水沟泥、工厂废水底泥、荷塘底泥、湖底泥等 5 种污泥混合 后驯化的活性污泥可作为棉秆沼气发酵的有效接种物, 其沼气发酵的日产气量和累积产气量均较 单种污泥驯化的接种物高。 关键词: 棉秆, 木质纤维素, 生物预处理, 活性污泥, 微生物多样性 Biological Pretreatment of Cotton Stalks and Domestication of Inocula in Biogas Fermentation BAI Yun LI Wei* CHEN Chun LIAO Peng (Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China) Abstract: A composite microbial system MEG with high efficient and stable degradation of cotton stalks was screened through the method of multigeneration selection. This microbial system was inoculated to the cotton stalks, and then stationarily treated for 7 days. Afterwards, biogas fermentation was conducted. Compared to the condition of non-pretreatment, the gas production increased by 25%. DGGE analysis showed that the diversity of bacterial community structure in the biogas fermentation system of cotton stalks was more abundant compared to the condition of non-pretreatment. At the same time, comparison in the effects of inocula obtained from 6 kinds of domestication methods on daily and cumulative of biogas production of cotton stalks was conducted. The results showed that the inocula domesticated from the mix- ture of 5 kinds of activated sludge from river bed mud, sewage ditch, industrial wastewater bed, pond bed, and lake bed could be acted as effective inocula for biogas fermentation of cotton stalks. The daily gas production and cumulative gas production were higher compared to the inocula domesticated from one kind of a