桃褐腐病菌（Moniliafructigena）原生质体制备.PDFVIP

微生物学通报 JAN 20, 2010, 37(1): 7177 Microbiology China © 2010 by Institute of Microbiology, CAS tongbao@ 研究报告 桃褐腐病菌(Monilia fructigena)原生质体制备 及再生条件 * * 薛伟 赵筱萌 刘素花 赵晓燕 刘正坪 (北京农学院 植物科学技术系 北京 102206) 摘 要: 以桃褐腐病菌(Monilia fructigena)为供试菌株, 研究了酶系组成、液体培养基、菌龄、酶 解温度、酶解时间对原生质体制备的影响, 以及等渗液、固体再生培养基、酶解时间对原生质体 再生的影响。结果表明: Fries(1/2)液体培养基培养24 h, 在 10 mg/mL 崩溃酶 + 5 mg/mL 纤维素酶 + 20 mg/mL 蜗牛酶 + 10 mg/mL 溶菌酶的混合酶液中28C 酶解4 h 为桃褐腐病菌原生质体制备的 最佳条件。采用液体再生涂布平板法, 以含 Ca2+ 的 STC 为等渗液的液体培养基和含蔗糖及 Ca2+ 的 Fries(1/2) 固体培养基为桃褐腐病菌原生质体再生的最佳条件。经过观察与测定, 再生菌株保持了原 有的培养性状和致病性, 接种桃果实后发病率为 100%。 关键词: 桃褐腐病菌, 原生质体, 再生 Protoplast Preparation and Regeneration of Monilia fructigena * XUE Wei ZHAO Xiao-Meng LIU Su-Hua ZHAO Xiao-Yan LIU Zheng-Ping* (Plant Science and Technology College, Beijing University of Agriculture, Beijing 102206, China) Abstract: The condition of protoplast preparation and regeneration of Monilia fructigena was studied in this research. The results showed that the composition of cell wall degradative enzymes, liquid medium, mycelial age, digesting temperature and time duration affected the preparation of protoplast. More proto- plast of M. fructigena were yielded when mycelia incubated in Fries(1/2) for 24 h were digested by en-