大理百合组织培养的研究.pdfVIP

Botanical Research 植物学研究, 2015, 4, 8-15 Published Online January 2015 in Hans. /journal/br /10.12677/br.2015.41002 Research on Tissue Culture of Lilium taliense Franch. Ning Shu, Wenjing Zhan, Yanni Liu, Guofeng Liu* Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan Hubei * Email: gfliu@ th th th Received: Dec. 17 , 2014; accepted: Jan. 6 , 2015; published: Jan. 20 , 2015 Copyright © 2015 by authors and Hans Publishers Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). /licenses/by/4.0/ Abstract Rapid propagation protocol of Lilium taliense Franch. by in vitro tissue culture was investigated. The results indicated that plant regeneration can be obtained from the bulb scales of L. taliense and a rapid propagation system was established efficiently. Among the media investigated, MS + 2.0 mg/L6-BA + 0.5 mg/LNAA + 3% sucrose is suitable for induction of adventitious bud and callus while MS + 0.5 mg/L6-BA + 0.1 mg/LNAA + 3% sucrose is better for induction of adventitious buds. The medium MS + 1.0 mg/L 6-BA + 0.1 mg/L NAA + 3% sucrose is proper for adventitious bud multiplication; MS + 1.0 mg/L NAA + 3% sucrose is optimum for bulblet formation from the adven- titious buds, while 1/2MS + NAA 0.3 mg/L + IBA0.1 mg/L + 12% sucrose is suitable for the growth of bulblets. Keywords Lilium taliense Franch., Tissue Culture, Rapid Propagation, Wild Lily 大理百合的组织培养研究 舒 宁，詹文静，刘燕妮，刘国锋* 华中农业大学园艺林学学院，园艺植物生物学教育部重点实验室，湖北 武汉 * Email: gfliu@ 收稿日期：2014年12月17 日；录用日期：2015年1月6 日；发布日期：20