多种方法提取苎麻组织基因组DNA质量比较.docVIP

多种方法提取苎麻组织基因组DNA质量比较 　　摘要:以苎麻[Boehmeria nivea (L.) Gaudich.]的纤维成熟期韧皮组织#65380;种子和根组织为试验材料,采用CTAB法#65380;SDS法和尿素法提取苎麻基因组DNA#65377;3种DNA提取方法比较,CTAB法提取的DNA质量优于其他两种方法;3种试验材料比较,韧皮组织提取的DNA质量最好,其次是种子和根组织#65377;种子提取的DNA浓度最高#65377;设计梯度试验分析不同研磨时间对韧皮组织和种子总DNA提取质量的影响,结果显示韧皮组织研磨4 min#65380;种子研磨6 min提取的总DNA质量较好#65377; 　　关键词:苎麻[Boehmeria nivea (L.) Gaudich.];基因组DNA;提取;组织;CTAB法;SDS法;尿素法 　　中图分类号:R281.4;Q7文献标识码:A文章编号:0439-8114(2014)11-2682-04 　　 　　Comparison of the Genomic DNA Extraction Methods From Different Ramie Tissues 　　 　　ZHANG Ping,XIA Dong-sheng,CAI Ya-jun,ZENG Qing-fu,WANG Jun 　　(Engineering Research Center for Cleaner Production of Textile Dying and printing Under Ministry of Education, 　　Wuhan Textile University,Wuhan 430073,China) 　　 　　Abatract: Extraction of high quality genomic DNA from Ramie tissue is the foundation for the study of molecular biology of ramie. In order to study ramie genome by some molecular test, such as PCR , In this study, CTAB, SDS and urea methods were used to extract the ramie genomic DNA from the phloem tissue of ramie fiber mature phase, seed and root tissue respectively. The DNA extraction effect of three different tissues and methods were compared by ultraviolet spectrophotometry and agarose electroforesis. The results showed that the extraction effect of CTAB method is best than the other two method; the extraction effect of the phloem tissue of ramie fiber mature phase is best in three different tissues, follow by the seed and root tissue. The seed is able to extract high quantity genomic DNA in three extraction methods. In addition we study different milling time of the phloem tissue and seeds to total DNA quality effect by used CTAB and SDS methods. The results showed that the phloem tissue grinding for 4min, seed tissue grinding for 6 minutes can get high quality DNA. 　　Key words:Boehmeria nivea(L.)Gaudich.;ramie genomic DNA;extraction; tissues; CTAB methods; SDS method; urea method 　　 　　基金项目:湖北省教育厅优秀中青年科技人才项目(;武